通过半巢式聚合酶链反应鉴定李斯特菌属菌种。
Identification of Listeria species by a semi-nested polymerase chain reaction.
作者信息
Manzano M, Cocolin L, Ferroni P, Gasparini V, Narduzzi D, Cantoni C, Comi G
机构信息
Dipartimento di Scienze degli Alimenti, Facoltà di Agraria, Università di Udine, Italy.
出版信息
Res Microbiol. 1996 Oct;147(8):637-40. doi: 10.1016/0923-2508(96)84020-4.
PMID:9157490
Abstract
Three primers derived from the lap gene were used to distinguish Listeria monocytogenes from L.innocua and other Listeria species. L. monocytogenes and L. innocua yielded a PCR product of 600 and 300 bp, respectively, whereas a typical pattern of three amplimers was observed with L. ivanovii, L. seeligeri and L. welshimeri.
摘要
从lap基因衍生的三种引物被用于区分单核细胞增生李斯特菌与无害李斯特菌及其他李斯特菌属物种。单核细胞增生李斯特菌和无害李斯特菌分别产生了600 bp和300 bp的PCR产物，而伊氏李斯特菌、斯氏李斯特菌和威氏李斯特菌则观察到三种扩增子的典型模式。
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