一种用于检测人工污染血液标本中单核细胞增生李斯特菌的巢式PCR方法。
A nested PCR method to detect Listeria monocytogenes in artificially contaminated blood specimens.
作者信息
Cocolin L, Manzano M, Cantoni C, Comi G
机构信息
Dipartimento di Scienze degli Alimenti, Facoltà di Agraria, Università di Udine, Italy.
出版信息
Res Microbiol. 1997 Jul-Aug;148(6):485-90. doi: 10.1016/S0923-2508(97)88346-5.
A nested PCR-based test was developed for the detection of Listeria monocytogenes in blood specimens from patients with listeriosis. Two pairs of oligonucleotide primers were designed to amplify a 1395-bp and a 453-bp fragment of the iap gene of L. monocytogenes. Amplified products were analysed with gel electrophoresis and stained with ethidium bromide. The PCR method described could be routinely used to diagnose listeriosis.
开发了一种基于巢式PCR的检测方法，用于检测李斯特菌病患者血液样本中的单核细胞增生李斯特菌。设计了两对寡核苷酸引物，以扩增单核细胞增生李斯特菌iap基因的1395 bp和453 bp片段。扩增产物用凝胶电泳分析，并用溴化乙锭染色。所描述的PCR方法可常规用于诊断李斯特菌病。
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