用脉冲场凝胶电泳法鉴别单核细胞增生李斯特菌、无害李斯特菌、伊氏李斯特菌和斯氏李斯特菌。
Differentiation of Listeria monocytogenes, Listeria innocua, Listeria ivanovii, and Listeria seeligeri by pulsed-field gel electrophoresis.
作者信息
Howard P J, Harsono K D, Luchansky J B
机构信息
Department of Food Microbiology and Toxicology, University of Wisconsin, Madison 53706-1187.
出版信息
Appl Environ Microbiol. 1992 Feb;58(2):709-12. doi: 10.1128/aem.58.2.709-712.1992.
Clamped homogeneous electric field analysis of Listeria DNA with ApaI, AscI, SmaI, or NotI revealed species- and serotype-specific differences in genomic fingerprints. Clamped homogeneous electric field analysis may prove useful, therefore, in epidemiologic studies. Also, the summation of individually sized AscI fragments of genomic DNA from L. monocytogenes serotype 4b 101M and Scott A indicated genome lengths of 2,925 and 3,046 kb, respectively.
用ApaI、AscI、SmaI或NotI对李斯特菌DNA进行钳位均匀电场分析，结果显示基因组指纹图谱存在物种和血清型特异性差异。因此，钳位均匀电场分析在流行病学研究中可能有用。此外，对单核细胞增生李斯特菌血清型4b 101M和斯科特A的基因组DNA中各个大小的AscI片段进行汇总分析，结果表明基因组长度分别为2925 kb和3046 kb。
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