• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

用脉冲场凝胶电泳法鉴别单核细胞增生李斯特菌、无害李斯特菌、伊氏李斯特菌和斯氏李斯特菌。

Differentiation of Listeria monocytogenes, Listeria innocua, Listeria ivanovii, and Listeria seeligeri by pulsed-field gel electrophoresis.

作者信息

Howard P J, Harsono K D, Luchansky J B

机构信息

Department of Food Microbiology and Toxicology, University of Wisconsin, Madison 53706-1187.

出版信息

Appl Environ Microbiol. 1992 Feb;58(2):709-12. doi: 10.1128/aem.58.2.709-712.1992.

DOI:10.1128/aem.58.2.709-712.1992
PMID:1610193
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC195308/
Abstract

Clamped homogeneous electric field analysis of Listeria DNA with ApaI, AscI, SmaI, or NotI revealed species- and serotype-specific differences in genomic fingerprints. Clamped homogeneous electric field analysis may prove useful, therefore, in epidemiologic studies. Also, the summation of individually sized AscI fragments of genomic DNA from L. monocytogenes serotype 4b 101M and Scott A indicated genome lengths of 2,925 and 3,046 kb, respectively.

摘要

用ApaI、AscI、SmaI或NotI对李斯特菌DNA进行钳位均匀电场分析，结果显示基因组指纹图谱存在物种和血清型特异性差异。因此，钳位均匀电场分析在流行病学研究中可能有用。此外，对单核细胞增生李斯特菌血清型4b 101M和斯科特A的基因组DNA中各个大小的AscI片段进行汇总分析，结果表明基因组长度分别为2925 kb和3046 kb。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6599/195308/8b184fbd352d/aem00043-0286-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6599/195308/c0b052c3ee08/aem00043-0286-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6599/195308/ef32713b6b11/aem00043-0286-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6599/195308/8b184fbd352d/aem00043-0286-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6599/195308/c0b052c3ee08/aem00043-0286-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6599/195308/ef32713b6b11/aem00043-0286-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6599/195308/8b184fbd352d/aem00043-0286-c.jpg

相似文献

1
Differentiation of Listeria monocytogenes, Listeria innocua, Listeria ivanovii, and Listeria seeligeri by pulsed-field gel electrophoresis.用脉冲场凝胶电泳法鉴别单核细胞增生李斯特菌、无害李斯特菌、伊氏李斯特菌和斯氏李斯特菌。
Appl Environ Microbiol. 1992 Feb;58(2):709-12. doi: 10.1128/aem.58.2.709-712.1992.
2
Construction of the temperature-sensitive vectors pLUCH80 and pLUCH88 for delivery of Tn917::NotI/SmaI and use of these vectors to derive a circular map of Listeria monocytogenes Scott A, a serotype 4b isolate.构建用于递送Tn917::NotI/SmaI的温度敏感型载体pLUCH80和pLUCH88，并使用这些载体绘制4b血清型单核细胞增生李斯特菌斯科特A菌株的环状图谱。
Appl Environ Microbiol. 1997 Sep;63(9):3480-7. doi: 10.1128/aem.63.9.3480-3487.1997.
3
Pulsed-field fingerprinting of listeriae: identification of genomic divisions for Listeria monocytogenes and their correlation with serovar.李斯特菌的脉冲场指纹图谱：单核细胞增生李斯特菌基因组分区的鉴定及其与血清型的相关性
Appl Environ Microbiol. 1994 Jul;60(7):2584-92. doi: 10.1128/aem.60.7.2584-2592.1994.
4
Pulsed-Field Gel Electrophoresis characterization of Listeria monocytogenes isolates from cheese manufacturing plants in São Paulo, Brazil.脉冲场凝胶电泳分析巴西圣保罗奶酪生产工厂中单核细胞增生李斯特菌的分离株。
Int J Food Microbiol. 2014 Mar 3;173:21-9. doi: 10.1016/j.ijfoodmicro.2013.12.018. Epub 2013 Dec 28.
5
An improved amplified fragment length polymorphism (AFLP) protocol for discrimination of Listeria isolates.一种用于区分李斯特菌分离株的改良扩增片段长度多态性（AFLP）方案。
Syst Appl Microbiol. 2003 Jun;26(2):236-44. doi: 10.1078/072320203322346083.
6
Polymorphism of Listeria monocytogenes and Listeria innocua strains isolated from short-ripened cheeses.从短期成熟奶酪中分离出的单核细胞增生李斯特菌和无害李斯特菌菌株的多态性。
J Appl Microbiol. 1998 Feb;84(2):255-62. doi: 10.1046/j.1365-2672.1998.00339.x.
7
Prevalence and antimicrobial resistance of Listeria species and molecular characterization of Listeria monocytogenes isolated from retail ready-to-eat foods.零售即食食品中李斯特菌的流行情况和耐药性以及单核细胞增生李斯特菌的分子特征。
FEMS Microbiol Lett. 2020 Feb 1;367(4). doi: 10.1093/femsle/fnaa006.
8
Isolation and pulsed-field gel electrophoresis typing of Listeria monocytogenes from modified atmosphere packaged fresh-cut vegetables collected in Ireland.从爱尔兰采集的气调包装鲜切蔬菜中分离单核细胞增生李斯特菌并进行脉冲场凝胶电泳分型
J Food Prot. 2006 Oct;69(10):2524-8. doi: 10.4315/0362-028x-69.10.2524.
9
Identification of Listeria species by a semi-nested polymerase chain reaction.通过半巢式聚合酶链反应鉴定李斯特菌属菌种。
Res Microbiol. 1996 Oct;147(8):637-40. doi: 10.1016/0923-2508(96)84020-4.
10
Differentiation of the major Listeria monocytogenes serovars by multiplex PCR.通过多重聚合酶链反应鉴别主要的单核细胞增生李斯特菌血清型。
J Clin Microbiol. 2004 Aug;42(8):3819-22. doi: 10.1128/JCM.42.8.3819-3822.2004.

引用本文的文献

1
Prevalence, Serotype Diversity, Genotype and Antibiotic Resistance of Listeria monocytogenes Isolated from Carcasses and Human in Korea.从韩国屠体和人体中分离出的单核细胞增生李斯特菌的流行率、血清型多样性、基因型及抗生素耐药性
Korean J Food Sci Anim Resour. 2018 Oct;38(5):851-865. doi: 10.5851/kosfa.2018.e5. Epub 2018 Oct 31.
2
Comparative phenotypic and molecular genetic profiling of wild Lactococcus lactis subsp. lactis strains of the L. lactis subsp. lactis and L. lactis subsp. cremoris genotypes, isolated from starter-free cheeses made of raw milk.对来自原料奶制作的无干酪乳杆菌干酪中分离的乳脂亚种乳杆菌和乳脂亚种克氏乳杆菌基因型的野生乳脂亚种乳杆菌菌株进行表型和分子遗传特征比较分析。
Appl Environ Microbiol. 2011 Aug;77(15):5324-35. doi: 10.1128/AEM.02991-10. Epub 2011 Jun 10.
3

本文引用的文献

1
Pulsed-Field Gel Electrophoresis of SmaI Digests of Lactococcal Genomic DNA, a Novel Method of Strain Identification.乳球菌基因组 DNA 的 SmaI 消化物的脉冲场凝胶电泳：一种新型的菌株鉴定方法。
Appl Environ Microbiol. 1990 Oct;56(10):3105-11. doi: 10.1128/aem.56.10.3105-3111.1990.
2
Purification, specific fragmentation, and separation of large DNA molecules.大型DNA分子的纯化、特异性片段化及分离
Methods Enzymol. 1987;155:449-67. doi: 10.1016/0076-6879(87)55030-3.
3
A physical map of the Escherichia coli K12 genome.大肠杆菌K12基因组的物理图谱。
Atomic force microscopy investigations of heterogeneities in the adhesion energies measured between pathogenic and non-pathogenic Listeria species and silicon nitride as they correlate to virulence and adherence.原子力显微镜研究致病性和非致病性李斯特菌与氮化硅之间测量的粘附能的非均质性，因为它们与毒力和粘附性有关。
Biofouling. 2011 May;27(5):543-59. doi: 10.1080/08927014.2011.584129.
4
Reliable and rapid identification of Listeria monocytogenes and Listeria species by artificial neural network-based Fourier transform infrared spectroscopy.基于人工神经网络的傅里叶变换红外光谱法对单核细胞增生李斯特菌和李斯特菌属进行可靠快速鉴定
Appl Environ Microbiol. 2006 Feb;72(2):994-1000. doi: 10.1128/AEM.72.2.994-1000.2006.
5
Rapid identification of Listeria species by using restriction fragment length polymorphism of PCR-amplified 23S rRNA gene fragments.利用聚合酶链反应扩增的23S rRNA基因片段的限制性片段长度多态性快速鉴定李斯特菌属菌种。
Appl Environ Microbiol. 2003 Nov;69(11):6386-92. doi: 10.1128/AEM.69.11.6386-6392.2003.
6
In vitro and in vivo invasiveness of different pulsed-field gel electrophoresis types of Listeria monocytogenes.不同脉冲场凝胶电泳类型的单核细胞增生李斯特菌的体外和体内侵袭性
Appl Environ Microbiol. 2002 Nov;68(11):5698-703. doi: 10.1128/AEM.68.11.5698-5703.2002.
7
Pulsed-field gel electrophoresis in differentiation of erysipelothrix species strains.脉冲场凝胶电泳用于红斑丹毒丝菌属菌株的鉴别
J Clin Microbiol. 2001 Nov;39(11):4032-6. doi: 10.1128/JCM.39.11.4032-4036.2001.
8
Construction of the temperature-sensitive vectors pLUCH80 and pLUCH88 for delivery of Tn917::NotI/SmaI and use of these vectors to derive a circular map of Listeria monocytogenes Scott A, a serotype 4b isolate.构建用于递送Tn917::NotI/SmaI的温度敏感型载体pLUCH80和pLUCH88，并使用这些载体绘制4b血清型单核细胞增生李斯特菌斯科特A菌株的环状图谱。
Appl Environ Microbiol. 1997 Sep;63(9):3480-7. doi: 10.1128/aem.63.9.3480-3487.1997.
9
The use of PCR ribotyping for typing strains of Listeria spp.利用聚合酶链反应核糖体分型法对李斯特菌属菌株进行分型
Eur J Epidemiol. 1995 Dec;11(6):665-73. doi: 10.1007/BF01720301.
10
Modified Listeria bacteriophage lysin genes (ply) allow efficient overexpression and one-step purification of biochemically active fusion proteins.修饰的李斯特菌噬菌体溶素基因（ply）可实现生物化学活性融合蛋白的高效过表达和一步纯化。
Appl Environ Microbiol. 1996 Aug;62(8):3057-60. doi: 10.1128/aem.62.8.3057-3060.1996.
Science. 1987 Jun 12;236(4807):1448-53. doi: 10.1126/science.3296194.
4
Restriction endonucleases for pulsed field mapping of bacterial genomes.用于细菌基因组脉冲场图谱分析的限制性内切酶
Nucleic Acids Res. 1987 Aug 11;15(15):5985-6005. doi: 10.1093/nar/15.15.5985.
5
Pulsed field gel electrophoresis.脉冲场凝胶电泳
Biotechniques. 1989 Jan;7(1):34-42.
6
Genome organization of the anaerobic pathogen Clostridium perfringens.厌氧病原菌产气荚膜梭菌的基因组组织
Proc Natl Acad Sci U S A. 1989 Sep;86(17):6676-80. doi: 10.1073/pnas.86.17.6676.
7
Towards a physical map of the Listeria chromosome: the pulsed field electrophoresis approach.构建李斯特菌染色体物理图谱：脉冲场电泳方法
Acta Microbiol Hung. 1989;36(2-3):193-7.
8
Cloning of the listeriolysin O gene and development of specific gene probes for Listeria monocytogenes.单核细胞增生李斯特菌溶血素O基因的克隆及该菌特异性基因探针的研制
Appl Environ Microbiol. 1990 Dec;56(12):3874-7. doi: 10.1128/aem.56.12.3874-3877.1990.
9
Detection of Listeria monocytogenes by using the polymerase chain reaction.利用聚合酶链反应检测单核细胞增生李斯特菌。
Appl Environ Microbiol. 1990 Sep;56(9):2930-2. doi: 10.1128/aem.56.9.2930-2932.1990.
10
A comparative study of clinical and food isolates of Listeria monocytogenes and related species.单核细胞增生李斯特菌及相关菌种临床分离株与食品分离株的比较研究
Epidemiol Infect. 1990 Oct;105(2):245-54. doi: 10.1017/s0950268800047853.