Santesteban E, Gibson J, Owen R J
Laboratory of Enteric Pathogens, Central Public Health Laboratory, London, United Kingdom.
Res Microbiol. 1996 Oct;147(8):641-9. doi: 10.1016/0923-2508(96)84021-6.
Flagellin gene (flaA) sequence polymorphisms were used to discriminate amongst 167 strains of Campylobacter jejuni serotype HS1 and the HS4 complex. Direct PCR of cell suspensions provided a rapid method for analysing DNase-negative strains, whereas purified DNA was necessary for the DNase-positive strains. Nine different PCR-RFLP patterns (genotypes) were identified by analysis with Hinfl and 12 with Ddel, giving a total of 19 combined flaA profile types. The most common combined fla types were H1D1 (35%) and H1D2 (20%) for serotype HS1, and H1D2 (23%) and H4D7 (43%) for serotype HS4. Comparison of flaA typing with other key subtyping methods for C. jejuni showed it to be less discriminatory than pulsed field gel electrophoretic (PFGE) profiling, but more so than ribotyping. Fla types provided a useful indication of strain diversity, but as some were conserved across different serotypes, ribotypes and PFGE types, the same fla type could not be used as the sole basis for grouping strains. We provide evidence for several distinct subgroups based on conserved multiple genomic criteria within the HS1 and HS4 strains, and conclude that monitoring of such subgroups could provide a novel basis for future epidemiological surveillance of C. jejuni.
鞭毛蛋白基因（flaA）序列多态性被用于区分167株空肠弯曲菌血清型HS1和HS4复合体。细胞悬液的直接PCR为分析DNase阴性菌株提供了一种快速方法，而对于DNase阳性菌株则需要纯化的DNA。通过Hinfl分析鉴定出9种不同的PCR-RFLP模式（基因型），通过Ddel分析鉴定出12种，总共产生19种组合的flaA谱型。血清型HS1最常见的组合fla类型是H1D1（35%）和H1D2（20%），血清型HS4是H1D2（23%）和H4D7（43%）。将flaA分型与空肠弯曲菌其他关键亚型分析方法进行比较，结果表明它的鉴别能力不如脉冲场凝胶电泳（PFGE）分析，但比核糖体分型更强。Fla类型为菌株多样性提供了有用的指示，但由于有些在不同血清型、核糖体分型和PFGE类型中是保守的，相同的fla类型不能作为菌株分组的唯一依据。我们基于HS1和HS4菌株内保守的多个基因组标准提供了几个不同亚组的证据，并得出结论，对这些亚组的监测可为未来空肠弯曲菌的流行病学监测提供新的依据。
