空肠弯曲菌血清型HS1和HS4的分子分型方案。
Molecular subtyping scheme for serotypes HS1 and HS4 of Campylobacter jejuni.
作者信息
Owen R J, Sutherland K, Fitzgerald C, Gibson J, Borman P, Stanley J
机构信息
National Collection of Type Cultures, Central Public Health Laboratory, London, United Kingdom.
出版信息
J Clin Microbiol. 1995 Apr;33(4):872-7. doi: 10.1128/jcm.33.4.872-877.1995.
Abstract
We describe a molecular subtyping scheme for two principal O (heat-stable [HS]) serotypes of Campylobacter jejuni, HS1 and the HS4 complex. A 16S rRNA gene-specific probe confirmed that almost all the C. jejuni strains had three copies of this gene, and strains could be assigned with complete typeability to 1 of 16 combined (Pst1 and HaeIII) 16S ribotypes. Macrorestriction profiles (mrps) consisting of up to 10 SmaI fragments from approximately 40 to approximately 480 kbp were resolved by pulsed-field gel electrophoresis (PFGE). There were 11 mrps among the HS1 strains and 9 mrps among HS4 strains which corresponded to valid types--they occurred in multiple isolates, hosts, places, and times. There were 14 additional single-strain mrp fingerprints in HS1 and 20 in HS4. PFGE exhibited complete typeability when formaldehyde fixation of cells was employed, and PFGE was generally more differential than ribotyping. The data presented elucidate a high-resolution genotypic subtyping scheme for these common subspecific phenotypes of C. jejuni, which is both coherent and efficient for epidemiological purposes.
摘要
我们描述了一种针对空肠弯曲菌两种主要O（热稳定[HS]）血清型HS1和HS4复合物的分子分型方案。一个16S rRNA基因特异性探针证实，几乎所有空肠弯曲菌菌株都有该基因的三个拷贝，并且菌株可以完全分型为16种组合（Pst1和HaeIII）16S核糖体分型中的一种。通过脉冲场凝胶电泳（PFGE）解析了由多达10个SmaI片段组成的宏观限制性图谱（mrps），片段大小约为40至约480 kbp。HS1菌株中有11种mrps，HS4菌株中有9种mrps，它们对应于有效类型——它们出现在多个分离株、宿主、地点和时间。HS1中还有14个单菌株mrp指纹图谱，HS4中有20个。当采用细胞甲醛固定时，PFGE表现出完全分型能力，并且PFGE通常比核糖体分型具有更高的鉴别力。所呈现的数据阐明了一种针对空肠弯曲菌这些常见亚种表型的高分辨率基因型分型方案，该方案对于流行病学目的而言既连贯又有效。
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