Slater E, Owen R J
Laboratory of Enteric Pathogens, Central Public Health Laboratory, London.
J Med Microbiol. 1998 Apr;47(4):353-7. doi: 10.1099/00222615-47-4-353.
High resolution molecular subtyping was applied to Campylobacter jejuni Penner heat-stable (HS) 11 isolates from human infections and other sources. Strains were genotyped by restriction fragment length polymorphism analysis involving PCR-based flagellin gene (flaA) profiling with HinfI and DdeI, and pulsed-field gel electrophoretic (PFGE) profiling with SmaI and KpnI. Fla-genes of the strains were highly conserved as most (95%) had the same fla-profile. PFGE analysis of SmaI digests was more discriminatory with 15 profile subtypes identified, although 36% of isolates had a common profile. The study showed that strains of C. jejuni HS11, unlike those of HS1 and the HS4 complex, were relatively homogeneous at the genomic level and that high resolution molecular techniques were essential for detailed epidemiological subtyping.
高分辨率分子亚型分析应用于空肠弯曲菌彭纳热稳定（HS）11分离株，这些分离株来自人类感染及其他来源。通过限制性片段长度多态性分析对菌株进行基因分型，包括基于PCR的鞭毛蛋白基因（flaA）用HinfI和DdeI进行分析，以及用SmaI和KpnI进行脉冲场凝胶电泳（PFGE）分析。菌株的Fla基因高度保守，因为大多数（95%）具有相同的Fla图谱。对SmaI消化产物的PFGE分析具有更高的鉴别力，鉴定出15种图谱亚型，尽管36%的分离株具有共同图谱。该研究表明，与HS1和HS4复合体的菌株不同，空肠弯曲菌HS11菌株在基因组水平上相对同质，并且高分辨率分子技术对于详细的流行病学亚型分析至关重要。
