DeGuise S, Bernier J, Dufresne M M, Martineau D, Beland P, Fournier M
TOXEN, Universite du Quebec a Montreal, Canada.
Vet Immunol Immunopathol. 1996 Mar;50(1-2):117-26. doi: 10.1016/0165-2427(95)05490-1.
A quantitative assay was developed to evaluate mitogen-induced lymphoblastic transformation in beluga whales (Delphinapterus leucas) using peripheral blood mononuclear cells, splenocytes and thymocytes. Optimal concentrations of four different mitogens (Con-A, PHA, LPS and PWM) were determined with the use of standard curves. Addition of human recombinant IL-2 (rhIL-2) after 48 h in culture with the different mitogens suggests that Con-A, PHA and PWM, but not LPS, stimulate T cells in belugas, as they do in other animal species. The addition of 2-mercaptoethanol did not enhance significantly the proliferation of cells stimulated by Con-A, PHA and LPS, while it did with the cells stimulated by PWM and those cultured without mitogen. The proliferative response of cells was suppressed when the culture medium was supplemented by beluga serum instead of fetal calf serum. This assay will be useful to assess the status of the immune functions in different populations of beluga whales as well for further in vitro immunotoxicological experiments.
开发了一种定量测定法，使用外周血单核细胞、脾细胞和胸腺细胞来评估白鲸（白鲸属）中丝裂原诱导的淋巴细胞转化。利用标准曲线确定了四种不同丝裂原（刀豆蛋白A、植物血凝素、脂多糖和美洲商陆有丝分裂原）的最佳浓度。在与不同丝裂原培养48小时后添加人重组白细胞介素-2（rhIL-2）表明，刀豆蛋白A、植物血凝素和美洲商陆有丝分裂原，而非脂多糖，能刺激白鲸中的T细胞，就像它们在其他动物物种中一样。添加2-巯基乙醇并没有显著增强刀豆蛋白A、植物血凝素和脂多糖刺激的细胞增殖，而对美洲商陆有丝分裂原刺激的细胞以及无丝裂原培养的细胞有增强作用。当培养基用白鲸血清而非胎牛血清补充时，细胞的增殖反应受到抑制。该测定法将有助于评估不同白鲸种群的免疫功能状态，也有助于进一步的体外免疫毒理学实验。
