De Guise S, Bernier J, Martineau D, Béland P, Fournier M
TOXEN, Université du Québec à Montréal, Canada.
Dev Comp Immunol. 1997 Sep-Oct;21(5):425-33. doi: 10.1016/s0145-305x(97)00021-9.
Widespread efforts are currently made to classify morphologically indistinguishable lymphocyte subpopulations in several species. In order to increase the knowledge in cetacean immunology, cross-reactivity of antibodies against bovine, human, ovine and mouse cell surface proteins was tested on beluga whale (Delphinapterus leucas) peripheral blood lymphocytes using flow cytometry. Anti-MHC class I and II as well as anti-CD2 reacted with virtually all peripheral blood lymphocytes. Anti-TCR gamma delta and anti-CD4 reacted with respectively 31% and 30% of peripheral blood lymphocytes. B lymphocytes were identified by an anti-surface IgM which was present on 6% of blood lymphocytes. Specificity of these antibodies was demonstrated by immunoprecipitation of beluga proteins with similar molecular weight to that of other species. These results could be useful for further immunotoxicological evaluation of highly versus mildly contaminated populations of belugas.
目前人们正在广泛努力对几个物种中形态上难以区分的淋巴细胞亚群进行分类。为了增加对鲸类免疫学的了解，利用流式细胞术在白鲸（白鲸属）外周血淋巴细胞上测试了针对牛、人、羊和小鼠细胞表面蛋白的抗体的交叉反应性。抗MHC I类和II类以及抗CD2与几乎所有外周血淋巴细胞发生反应。抗TCRγδ和抗CD4分别与31%和30%的外周血淋巴细胞发生反应。B淋巴细胞通过抗表面IgM鉴定，其存在于6%的血液淋巴细胞上。通过对白鲸蛋白进行免疫沉淀，证明了这些抗体的特异性，白鲸蛋白的分子量与其他物种相似。这些结果可能有助于对白鲸高度污染与轻度污染群体进行进一步的免疫毒理学评估。
