Gmoshinskiĭ I V, Mazo V K, Zorin S N
Vopr Pitan. 1996(1):3-6.
Rats received dietary supplement of milk whey concentrate (KSP) being manufactured from skimmed milk by addition of apple pectin (Pec), KSP ultrafiltrated fractions and pure Pec during 21 day. A part of animals were sensitized i/p with chicken ovalbumin and finally subjected to systemic anaphylaxis (SA) by i/v antigen challenge. Gastrointestinal barrier macromolecular permeability (MP) for polyethylene glycol 4000 (PEG-4000) was measured by its urinary excretion after an intragastric load. MP increased significantly in nonsensitized rats after feeding with KSP and its high molecular (HM) fraction but not after feeding with low molecular (LM) KSP fraction compared to NaCl fed group. Feeding with Pec dramatically (5-fold) increased permeability. On the contrary the permeability decreased in sensitized rats subjected to SA when fed with KSP, its LM and particularly HM fraction the later group permeability being normalized down to normal value found in nonsensitized animals. It's concluded that KSP whey protein may be recommended for incorporation into specialized foods for patients with impaired gastrointestinal function.
大鼠在21天内接受了由脱脂牛奶添加苹果果胶（Pec）制成的乳清浓缩物（KSP）、KSP超滤级分和纯Pec的膳食补充。一部分动物经腹腔注射鸡卵清蛋白致敏，最后通过静脉注射抗原激发进行全身过敏反应（SA）。通过胃内负荷后聚乙二醇4000（PEG - 4000）的尿排泄来测量胃肠道屏障大分子通透性（MP）。与喂食氯化钠的组相比，喂食KSP及其高分子（HM）级分后，未致敏大鼠的MP显著增加，但喂食低分子（LM）KSP级分后未增加。喂食Pec可使通透性显著（5倍）增加。相反，在接受SA的致敏大鼠中，喂食KSP、其LM级分尤其是HM级分后，通透性降低，后一组的通透性恢复到未致敏动物的正常水平。结论是，KSP乳清蛋白可推荐用于胃肠道功能受损患者的特殊食品中。
