Mercier G, Burckel A, Lucotte G
Burckel Laboratory, Paris, France.
Mol Cell Probes. 1997 Apr;11(2):89-94. doi: 10.1006/mcpr.1996.0090.
A polymerase chain reaction (PCR) assay was developed for the detection of Borrelia burgdorferi-specific DNA in the urine of patients with erythema migrans (EM). The target for the PCR was a specific region of the flagellin gene, and DNA was extracted from urine by Chelex resin. The detection limit was 1-10 genomes of B. burgdorferi, B. garinii or B. afzelii. A prospective study was performed with 12 consecutively diagnosed patients with EM, to evaluate the PCR assay on clinical samples. Borrelia burgdorferi-specific DNA could be detected in urine specimens from the 12 patients with EM before antibiotic therapy. Five weeks after therapy all the patients were negative by PCR of urine. Results of the present study confirm that the described PCR assay is sensitive and that this sort of test allows monitoring of the efficacy of therapy in patients with early Lyme borreliosis.
开发了一种聚合酶链反应（PCR）检测方法，用于检测游走性红斑（EM）患者尿液中的伯氏疏螺旋体特异性DNA。PCR的靶标是鞭毛蛋白基因的一个特定区域，DNA通过Chelex树脂从尿液中提取。检测限为伯氏疏螺旋体、伽氏疏螺旋体或阿氏疏螺旋体的1-10个基因组。对12例连续诊断为EM的患者进行了一项前瞻性研究，以评估该PCR检测方法对临床样本的效果。在抗生素治疗前，12例EM患者的尿液标本中均可检测到伯氏疏螺旋体特异性DNA。治疗五周后，所有患者的尿液PCR检测均为阴性。本研究结果证实，所描述的PCR检测方法具有敏感性，并且这种检测方法可用于监测早期莱姆病患者的治疗效果。
