培养及聚合酶链反应对游走性红斑病因诊断的敏感性

Sensitivity of culture and polymerase chain reaction for the etiologic diagnosis of erythema migrans.

作者信息

Zore Anamarija, Ruzić-Sabljić Eva, Maraspin Vera, Cimperman Joze, Lotric-Furlan Stanka, Pikelj Andreja, Jurca Tomaz, Logar Mateja, Strle Franc

机构信息

Institute of Microbiology and Immunology, Medical Faculty, University of Ljubljana, Slovenia.

出版信息

Wien Klin Wochenschr. 2002 Jul 31;114(13-14):606-9.

PMID:12422609

Abstract

Skin biopsy samples from 150 patients with typical cutaneous manifestation of Lyme borreliosis, erythema migrans, were cultivated for the presence of Borrelia burgdorferi sensu lato in modified Kelly Pettenkofer (MKP) medium and analysed with two different polymerase chain reactions using either flagellin or nested OspA primers. Cultivation was successful in 75 of 150 (50%) skin samples. Out of 70 strains that were typed using PFGE, B. afzelii was identified in 60 (86%), B. garinii in 10 (14%) specimens, while no B. burgdorferi sensu stricto strains were found. B. burgdorferi sensu lato DNA was detected with polymerase chain reaction in 28% and 61% of skin samples, using flagellin and nested OspA primers, respectively. Concordant results in all three procedures employed in the present study were found in 62 (41%) specimens: 25/150 (17%) were positive with all three methods and 37/150 (25%) samples were completely negative.

摘要

从150例患有莱姆病典型皮肤表现——游走性红斑的患者身上采集皮肤活检样本，在改良的凯利-佩滕科费尔（MKP）培养基中培养以检测伯氏疏螺旋体狭义种的存在，并使用鞭毛蛋白或巢式OspA引物通过两种不同的聚合酶链反应进行分析。150份皮肤样本中有75份（50%）培养成功。在使用脉冲场凝胶电泳（PFGE）分型的70株菌株中，60份（86%）鉴定为阿氏疏螺旋体，10份（14%）标本鉴定为伽氏疏螺旋体，未发现狭义伯氏疏螺旋体菌株。分别使用鞭毛蛋白和巢式OspA引物，通过聚合酶链反应在28%和61%的皮肤样本中检测到伯氏疏螺旋体狭义种DNA。本研究采用的所有三种检测程序结果一致的标本有62份（41%）：25/150（17%）三种方法均为阳性，37/150（25%）样本均为完全阴性。