de la Torre F, García J C, Martínez A, Martínez J, Palacios R
Unidad de Alergia, Hospital Nuestra. Sra. de Candelaria, Tenerife, Spain.
J Investig Allergol Clin Immunol. 1997 Mar-Apr;7(2):83-9.
Pollen proteins, most frequently from Compositae plants, and glandular enzymes from Hymenoptera insects are the only honey allergens involved in every case of honey sensitization reported so far. Surprisingly, we found three patients, all showing the same pattern of honey sensitization with clinical history, cutaneous test and specific IgE clearly positive to honey crude extract but not to the aforementioned components. IgE binding bands, mainly at 54, 46, 17 and 16 kDa, were recognized by the sera of the three patients on blots following electrophoretic separation of a local honey extract under dissociating conditions. Attempts to identify these proteins with the most common honey allergens by means of SDS-PAGE immunoblotting failed, leaving open the question on the origin of protein allergens in cases of honey allergy. Thus, if bee-derived components are not proven to be involved in honey sensitization, other protein sources, such as nectar, should not be disregarded.
花粉蛋白（最常见于菊科植物）和膜翅目昆虫的腺体酶是迄今为止报告的每一例蜂蜜致敏病例中唯一涉及的蜂蜜过敏原。令人惊讶的是，我们发现三名患者，他们在临床病史、皮肤试验以及对蜂蜜粗提物特异性IgE明显呈阳性但对上述成分呈阴性方面均表现出相同的蜂蜜致敏模式。在解离条件下对当地蜂蜜提取物进行电泳分离后，三名患者的血清在印迹上识别出主要位于54、46、17和16 kDa的IgE结合带。通过SDS-PAGE免疫印迹法将这些蛋白质与最常见的蜂蜜过敏原进行鉴定的尝试失败了，这使得蜂蜜过敏病例中蛋白质过敏原的来源问题悬而未决。因此，如果未证明蜜蜂来源的成分与蜂蜜致敏有关，那么其他蛋白质来源，如花蜜，也不应被忽视。
