Bottaro G, Volta U, Spina M, Rotolo N, Sciacca A, Musumeci S
Department of Pediatrics, University of Catania, Italy.
J Pediatr Gastroenterol Nutr. 1997 May;24(5):559-62. doi: 10.1097/00005176-199705000-00013.
We carried out a study of the antibody pattern in 50 celiac children [34 females (F) and 16 males (M); F/M, 2.1], ages 7 months-15 years, compared with that in 25 control subjects (13 females and 12 males) of the same age.
IgA and IgG antigliadin antibodies (AGA) were determined with an enzyme-linked immunosorbent assay technique. IgA anti-R1-reticulin antibodies (ARA) and IgA antiendomysium antibodies (EmA) were determined with the fluorescein isothiocyanate-conjugate-labeled anti-human immunoglobulin technique. To compare sensitivity and specificity, EmA were identified using monkey esophagus and human umbilical cord as substrates.
While AGA (IgA and IgG) showed a high sensitivity but a low specificity, ARA showed a high specificity but a low sensitivity. Data on EmA showed a high sensitivity and specificity with both tissue sections, with monkey esophagus being more sensitive (96%) and umbilical cord more specific (100%).
Our results confirm the importance of celiac disease-related antibodies in identifying celiac children. Moreover, the easy availability of human umbilical cord indicates that it would be proper to use this tissue as substrate, instead of monkey esophagus, for EmA search in the future.
我们对50名乳糜泻患儿[34名女性（F）和16名男性（M）；女性/男性，2.1]进行了抗体模式研究，这些患儿年龄在7个月至15岁之间，并与25名同年龄的对照受试者（13名女性和12名男性）进行了比较。
采用酶联免疫吸附测定技术测定IgA和IgG抗麦醇溶蛋白抗体（AGA）。采用异硫氰酸荧光素共轭标记的抗人免疫球蛋白技术测定IgA抗R1 - 网状纤维抗体（ARA）和IgA抗肌内膜抗体（EmA）。为比较敏感性和特异性，以猴食管和人脐带为底物鉴定EmA。
虽然AGA（IgA和IgG）显示出高敏感性但低特异性，ARA显示出高特异性但低敏感性。EmA的数据显示，在两种组织切片上均具有高敏感性和特异性，猴食管更敏感（96%），脐带更具特异性（100%）。
我们的结果证实了乳糜泻相关抗体在识别乳糜泻患儿中的重要性。此外，人脐带容易获取，这表明未来在进行EmA检测时，使用这种组织作为底物而非猴食管是合适的。
