Iwasaki Y, Ikeda K, Shiojima T, Kobayashi T, Tagaya N, Kinoshita M
Fourth Department of Internal Medicine, Toho University Ohashi Hospital, Tokyo, Japan.
Neurol Res. 1996 Apr;18(2):168-70. doi: 10.1080/01616412.1996.11740397.
It has been reported that both the monoamine oxidase inhibitor, deprenyl and the dopamine receptor agonist, pergolide have neuroprotective actions. To investigate the effect of deprenyl and pergolide on axotomized motor neuron death, we examined the survival of spinal motor neurons after sciatic nerve transection in the neonatal rats. Newborn rats were anesthetized with hypothermia. Sciatic nerve was cut near the obturator tendon in the left thigh. Animals were then treated daily with deprenyl (10 mg kg(-1)), pergolide (5 mg kg(-1)), or PBS for 14 days with intraperitoneal injections in a blind fashion. After the treatment, the number of spinal motor neurons in the L 4-6 was counted. There was approximately a 50% loss of spinal motor neurons in PBS-treated group. By contrast, both deprenyl and pergolide prevents spinal motor neuron death after axotomy Co-administration of deprenyl and pergolide is more effective than either agent alone but not significant. These findings are consistent with the idea that deprenyl and pergolide are survival factors for developing spinal motor neurons.
据报道，单胺氧化酶抑制剂司来吉兰和多巴胺受体激动剂培高利特都具有神经保护作用。为了研究司来吉兰和培高利特对轴突切断后运动神经元死亡的影响，我们检测了新生大鼠坐骨神经横断后脊髓运动神经元的存活情况。新生大鼠用低温麻醉。在左大腿闭孔肌腱附近切断坐骨神经。然后对动物进行盲法腹腔注射，每日给予司来吉兰（10 mg kg(-1)）、培高利特（5 mg kg(-1)）或磷酸盐缓冲液（PBS），持续14天。治疗后，计数L 4 - 6节段的脊髓运动神经元数量。在PBS治疗组中，脊髓运动神经元大约损失了50%。相比之下，司来吉兰和培高利特均可防止轴突切断后脊髓运动神经元的死亡。司来吉兰和培高利特联合使用比单独使用任何一种药物更有效，但差异不显著。这些发现与司来吉兰和培高利特是发育中的脊髓运动神经元存活因子的观点一致。
