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趋化因子通过多种信号通路诱导盘基网柄菌中ERK2的酪氨酸磷酸化。

Chemoattractants induce tyrosine phosphorylation of ERK2 in Dictyostelium discoideum by diverse signalling pathways.

作者信息

Kosaka C, Pears C J

机构信息

Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, U.K.

出版信息

Biochem J. 1997 May 15;324 ( Pt 1)(Pt 1):347-52. doi: 10.1042/bj3240347.

DOI:10.1042/bj3240347
PMID:9164876
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1218436/
Abstract

Two homologues of mitogen-activated protein kinases have been identified in Dictyostelium discoideum (ERK1 and EKR2). We here demonstrate transient tyrosine phosphorylation of ERK2 in response to the chemoattractants cAMP and folic acid that correlates with activity. To investigate the signalling pathways, we studied the response in strains with altered cAMP-dependent protein kinase (PKA) status. The degree of cAMP-induced ERK2 tyrosine phosphorylation was increased in cells overexpressing PKA activity but no such increase was observed in the response to folic acid. Our observations suggest that cAMP-induced ERK2 tyrosine phosphorylation is positively modulated by a PKA-regulated step which is not involved in the response to folic acid, suggesting the presence of diverse signalling pathways leading to ERK2 activation.

摘要

在盘基网柄菌中已鉴定出两种丝裂原活化蛋白激酶的同源物（ERK1和EKR2）。我们在此证明，响应趋化剂cAMP和叶酸时，ERK2会发生瞬时酪氨酸磷酸化，且这种磷酸化与活性相关。为了研究信号通路，我们研究了cAMP依赖性蛋白激酶（PKA）状态改变的菌株的反应。在过表达PKA活性的细胞中，cAMP诱导的ERK2酪氨酸磷酸化程度增加，但在对叶酸的反应中未观察到这种增加。我们的观察结果表明，cAMP诱导的ERK2酪氨酸磷酸化受到PKA调节步骤的正向调节，该步骤不参与对叶酸的反应，这表明存在导致ERK2激活的多种信号通路。

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本文引用的文献

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Signaling through chemoattractant receptors in Dictyostelium.盘基网柄菌中趋化因子受体介导的信号传导
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Dual role of cAMP and involvement of both G-proteins and ras in regulation of ERK2 in Dictyostelium discoideum.环磷酸腺苷（cAMP）的双重作用以及G蛋白和ras在盘基网柄菌中对细胞外信号调节激酶2（ERK2）调控中的参与
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MAP kinase kinase kinase, MAP kinase kinase and MAP kinase.丝裂原活化蛋白激酶激酶激酶、丝裂原活化蛋白激酶激酶和丝裂原活化蛋白激酶。
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The G alpha subunit G alpha 4 couples to pterin receptors and identifies a signaling pathway that is essential for multicellular development in Dictyostelium.Gα亚基Gα4与蝶呤受体偶联，并确定了一条对盘基网柄菌多细胞发育至关重要的信号通路。
Proc Natl Acad Sci U S A. 1994 Oct 25;91(22):10566-70. doi: 10.1073/pnas.91.22.10566.
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Mol Cell Biol. 1994 Oct;14(10):6996-7012. doi: 10.1128/mcb.14.10.6996-7012.1994.
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A MAP kinase necessary for receptor-mediated activation of adenylyl cyclase in Dictyostelium.一种对盘基网柄菌中受体介导的腺苷酸环化酶激活所必需的丝裂原活化蛋白激酶。
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The G protein beta subunit is essential for multiple responses to chemoattractants in Dictyostelium.G蛋白β亚基对于盘基网柄菌对趋化因子的多种反应至关重要。
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