趋化因子通过多种信号通路诱导盘基网柄菌中ERK2的酪氨酸磷酸化。
Chemoattractants induce tyrosine phosphorylation of ERK2 in Dictyostelium discoideum by diverse signalling pathways.
作者信息
Kosaka C, Pears C J
机构信息
Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, U.K.
出版信息
Biochem J. 1997 May 15;324 ( Pt 1)(Pt 1):347-52. doi: 10.1042/bj3240347.
Abstract
Two homologues of mitogen-activated protein kinases have been identified in Dictyostelium discoideum (ERK1 and EKR2). We here demonstrate transient tyrosine phosphorylation of ERK2 in response to the chemoattractants cAMP and folic acid that correlates with activity. To investigate the signalling pathways, we studied the response in strains with altered cAMP-dependent protein kinase (PKA) status. The degree of cAMP-induced ERK2 tyrosine phosphorylation was increased in cells overexpressing PKA activity but no such increase was observed in the response to folic acid. Our observations suggest that cAMP-induced ERK2 tyrosine phosphorylation is positively modulated by a PKA-regulated step which is not involved in the response to folic acid, suggesting the presence of diverse signalling pathways leading to ERK2 activation.
摘要
在盘基网柄菌中已鉴定出两种丝裂原活化蛋白激酶的同源物（ERK1和EKR2）。我们在此证明，响应趋化剂cAMP和叶酸时，ERK2会发生瞬时酪氨酸磷酸化，且这种磷酸化与活性相关。为了研究信号通路，我们研究了cAMP依赖性蛋白激酶（PKA）状态改变的菌株的反应。在过表达PKA活性的细胞中，cAMP诱导的ERK2酪氨酸磷酸化程度增加，但在对叶酸的反应中未观察到这种增加。我们的观察结果表明，cAMP诱导的ERK2酪氨酸磷酸化受到PKA调节步骤的正向调节，该步骤不参与对叶酸的反应，这表明存在导致ERK2激活的多种信号通路。
相似文献
1
Chemoattractants induce tyrosine phosphorylation of ERK2 in Dictyostelium discoideum by diverse signalling pathways.趋化因子通过多种信号通路诱导盘基网柄菌中ERK2的酪氨酸磷酸化。
Biochem J. 1997 May 15;324 ( Pt 1)(Pt 1):347-52. doi: 10.1042/bj3240347.
2
Negative influence of RasG on chemoattractant-induced ERK2 phosphorylation in Dictyostelium.RasG对盘基网柄菌中趋化因子诱导的ERK2磷酸化的负面影响。
Biochim Biophys Acta. 1998 Mar 12;1402(1):1-5. doi: 10.1016/s0167-4889(98)00010-x.
3
4
The Dictyostelium MAP kinase DdERK2 functions as a cytosolic protein in complexes with its potential substrates in chemotactic signal transduction.盘基网柄菌丝裂原活化蛋白激酶DdERK2在趋化信号转导中与其潜在底物形成复合物，作为一种胞质蛋白发挥作用。
Biochem Biophys Res Commun. 1998 Mar 6;244(1):149-55. doi: 10.1006/bbrc.1997.8118.
5
Activation of the mitogen-activated protein kinase ERK2 by the chemoattractant folic acid in Dictyostelium.趋化因子叶酸在盘基网柄菌中对丝裂原活化蛋白激酶ERK2的激活作用。
J Biol Chem. 1997 Sep 19;272(38):23690-5. doi: 10.1074/jbc.272.38.23690.
6
The Dictyostelium mitogen-activated protein kinase ERK2 is regulated by Ras and cAMP-dependent protein kinase (PKA) and mediates PKA function.盘基网柄菌促分裂原活化蛋白激酶ERK2受Ras和环磷酸腺苷依赖性蛋白激酶（PKA）调控，并介导PKA功能。
J Biol Chem. 1997 Feb 14;272(7):3883-6. doi: 10.1074/jbc.272.7.3883.
7
MAP kinases have different functions in Dictyostelium G protein-mediated signaling.MAP 激酶在 Dictyostelium G 蛋白介导的信号转导中具有不同的功能。
Cell Signal. 2010 May;22(5):836-47. doi: 10.1016/j.cellsig.2010.01.008. Epub 2010 Jan 14.
8
The Dictyostelium MAP kinase kinase DdMEK1 regulates chemotaxis and is essential for chemoattractant-mediated activation of guanylyl cyclase.盘基网柄菌丝裂原活化蛋白激酶激酶DdMEK1调节趋化作用，对于趋化因子介导的鸟苷酸环化酶激活至关重要。
EMBO J. 1997 Jul 16;16(14):4317-32. doi: 10.1093/emboj/16.14.4317.
9
Comparative analysis of chemotaxis in Dictyostelium using a radial bioassay method: protein tyrosine kinase activity is required for chemotaxis to folate but not to cAMP.采用放射状生物测定法对盘基网柄菌趋化性的比较分析：趋化至叶酸而非环磷酸腺苷需要蛋白质酪氨酸激酶活性。
Cell Signal. 1995 Jul;7(5):481-9. doi: 10.1016/0898-6568(95)00016-i.
10
Biochemical responses to chemoattractants in Dictyostelium: ligand-receptor interactions and downstream kinase activation.盘基网柄菌对趋化剂的生化反应：配体-受体相互作用及下游激酶激活
Methods Mol Biol. 2009;571:271-81. doi: 10.1007/978-1-60761-198-1_18.
引用本文的文献
1
Dictyostelium Erk2 is an atypical MAPK required for chemotaxis.粘菌 Erk2 是一种非典型的丝裂原活化蛋白激酶，对于趋化作用是必需的。
Cell Signal. 2018 Jun;46:154-165. doi: 10.1016/j.cellsig.2018.03.006. Epub 2018 Mar 15.
2
A polycystin-type transient receptor potential (Trp) channel that is activated by ATP.一种由三磷酸腺苷（ATP）激活的多囊蛋白型瞬时受体电位（Trp）通道。
Biol Open. 2017 Feb 15;6(2):200-209. doi: 10.1242/bio.020685.
3
The Dictyostelium prestalk inducer differentiation-inducing factor-1 (DIF-1) triggers unexpectedly complex global phosphorylation changes.盘基网柄菌前柄细胞诱导物分化诱导因子-1（DIF-1）引发了意想不到的复杂全局磷酸化变化。
Mol Biol Cell. 2015 Feb 15;26(4):805-20. doi: 10.1091/mbc.E14-08-1319. Epub 2014 Dec 17.
4
Arrestins function in cAR1 GPCR-mediated signaling and cAR1 internalization in the development of Dictyostelium discoideum.抑制蛋白在盘基网柄菌发育过程中的cAR1 G蛋白偶联受体介导的信号传导和cAR1内化中发挥作用。
Mol Biol Cell. 2014 Oct 15;25(20):3210-21. doi: 10.1091/mbc.E14-03-0834. Epub 2014 Aug 20.
5
MAPKs in development: insights from Dictyostelium signaling pathways.发育过程中的丝裂原活化蛋白激酶：来自盘基网柄菌信号通路的见解。
Biomol Concepts. 2011 Apr 1;2(1-2):39-46. doi: 10.1515/BMC.2011.004.
6
MAP kinases have different functions in Dictyostelium G protein-mediated signaling.MAP 激酶在 Dictyostelium G 蛋白介导的信号转导中具有不同的功能。
Cell Signal. 2010 May;22(5):836-47. doi: 10.1016/j.cellsig.2010.01.008. Epub 2010 Jan 14.
7
The Galpha4 G protein subunit interacts with the MAP kinase ERK2 using a D-motif that regulates developmental morphogenesis in Dictyostelium.Gα4 G蛋白亚基通过一个D基序与丝裂原活化蛋白激酶ERK2相互作用，该D基序调节盘基网柄菌的发育形态发生。
Dev Biol. 2009 Nov 15;335(2):385-95. doi: 10.1016/j.ydbio.2009.09.011. Epub 2009 Sep 15.
8
MPL1, a novel phosphatase with leucine-rich repeats, is essential for proper ERK2 phosphorylation and cell motility.MPL1是一种具有富含亮氨酸重复序列的新型磷酸酶，对ERK2的正常磷酸化和细胞运动至关重要。
Eukaryot Cell. 2008 Jun;7(6):958-66. doi: 10.1128/EC.00442-07. Epub 2008 Apr 11.
9
Nonadaptive regulation of ERK2 in Dictyostelium: implications for mechanisms of cAMP relay.盘基网柄菌中ERK2的非适应性调节：对cAMP信号传递机制的启示
Mol Biol Cell. 2006 Oct;17(10):4220-7. doi: 10.1091/mbc.e06-05-0376. Epub 2006 Jul 26.
10
Cytoskeletal regulation by Dictyostelium Ras subfamily proteins.盘基网柄菌Ras亚家族蛋白对细胞骨架的调控
J Muscle Res Cell Motil. 2002;23(7-8):729-36. doi: 10.1023/a:1024471527153.
本文引用的文献
1
Signaling through chemoattractant receptors in Dictyostelium.盘基网柄菌中趋化因子受体介导的信号传导
Trends Genet. 1996 Feb;12(2):52-7. doi: 10.1016/0168-9525(96)81400-4.
2
3
Seven helix chemoattractant receptors transiently stimulate mitogen-activated protein kinase in Dictyostelium. Role of heterotrimeric G proteins.七螺旋趋化因子受体在盘基网柄菌中短暂刺激丝裂原活化蛋白激酶。异源三聚体G蛋白的作用。
J Biol Chem. 1996 Feb 16;271(7):3351-4. doi: 10.1074/jbc.271.7.3351.
4
Extracellular cAMP is sufficient to restore developmental gene expression and morphogenesis in Dictyostelium cells lacking the aggregation adenylyl cyclase (ACA).细胞外的环磷酸腺苷（cAMP）足以恢复缺乏聚集型腺苷酸环化酶（ACA）的盘基网柄菌细胞中的发育基因表达和形态发生。
Genes Dev. 1993 Nov;7(11):2172-80. doi: 10.1101/gad.7.11.2172.
5
MAP kinase kinase kinase, MAP kinase kinase and MAP kinase.丝裂原活化蛋白激酶激酶激酶、丝裂原活化蛋白激酶激酶和丝裂原活化蛋白激酶。
Curr Opin Genet Dev. 1994 Feb;4(1):82-9. doi: 10.1016/0959-437x(94)90095-7.
6
The G alpha subunit G alpha 4 couples to pterin receptors and identifies a signaling pathway that is essential for multicellular development in Dictyostelium.Gα亚基Gα4与蝶呤受体偶联，并确定了一条对盘基网柄菌多细胞发育至关重要的信号通路。
Proc Natl Acad Sci U S A. 1994 Oct 25;91(22):10566-70. doi: 10.1073/pnas.91.22.10566.
7
Identification and functional analysis of a developmentally regulated extracellular signal-regulated kinase gene in Dictyostelium discoideum.盘基网柄菌中一个受发育调控的细胞外信号调节激酶基因的鉴定与功能分析。
Mol Cell Biol. 1994 Oct;14(10):6996-7012. doi: 10.1128/mcb.14.10.6996-7012.1994.
8
Cyclic AMP activates the mitogen-activated protein kinase cascade in PC12 cells.环磷酸腺苷激活PC12细胞中的丝裂原活化蛋白激酶级联反应。
J Biol Chem. 1994 Feb 25;269(8):6207-14.
9
A MAP kinase necessary for receptor-mediated activation of adenylyl cyclase in Dictyostelium.一种对盘基网柄菌中受体介导的腺苷酸环化酶激活所必需的丝裂原活化蛋白激酶。
J Cell Biol. 1995 Feb;128(3):405-13. doi: 10.1083/jcb.128.3.405.
10
The G protein beta subunit is essential for multiple responses to chemoattractants in Dictyostelium.G蛋白β亚基对于盘基网柄菌对趋化因子的多种反应至关重要。
J Cell Biol. 1995 Jun;129(6):1667-75. doi: 10.1083/jcb.129.6.1667.
Zotero 插件