Gupta A, Bazer F W, Jaeger L A
Department of Veterinary Anatomy and Public Health, Institute of Biosciences and Technology, Texas A&M University, College Station 77843, USA.
Biol Reprod. 1997 Jun;56(6):1527-36. doi: 10.1095/biolreprod56.6.1527.
Immunohistochemical techniques were used to localize acidic and basic fibroblast growth factor (FGF) polypeptides in porcine uterine and conceptus tissues collected on Days 10 through 14 of gestation, which is the peri-implantation period, and in uterine tissues collected on the same days of the estrous cycle. Our results demonstrate differential expression of acidic and basic FGF (aFGF, bFGF) in porcine uterine and conceptus tissues. Localization of these peptides in the uterus of cycling and pregnant pigs was different from that reported for other species, suggesting species-specific roles for FGFs in early pregnancy. Increases in both cytoplasmic and nuclear bFGF immunostaining were detected in uterine luminal and glandular epithelial cells from Days 10 to 14 of gestation but not in the uterine epithelium of cycling pigs. Acidic FGF immunostaining was not detected in luminal or glandular epithelium of either cycling or pregnant uterine tissue; however, differential stromal staining was observed. Uterine tissues collected from pigs on Days 10-14 of the estrous cycle had diffuse aFGF immunostaining throughout the stroma. During early pregnancy, however, intense aFGF immunostaining was concentrated around the glandular epithelial and below the luminal epithelial cells as gestation progressed. Basic FGF, but not aFGF, was detected in porcine conceptuses collected during the peri-implantation period (Days 10-14 of gestation). Although both acidic and basic FGF belong to the same family of proteins, results of the present study indicate that they likely play different roles in uterine function and conceptus development in pigs.
免疫组织化学技术用于在妊娠第10至14天（即围植入期）收集的猪子宫和孕体组织中，以及在发情周期相同天数收集的子宫组织中定位酸性和碱性成纤维细胞生长因子（FGF）多肽。我们的结果表明，酸性和碱性FGF（aFGF、bFGF）在猪子宫和孕体组织中存在差异表达。这些肽在发情周期和怀孕母猪子宫中的定位与其他物种的报道不同，表明FGFs在早期妊娠中具有物种特异性作用。在妊娠第10至14天，子宫腔和腺上皮细胞的细胞质和细胞核bFGF免疫染色均增加，但发情周期母猪的子宫上皮细胞中未增加。在发情周期或怀孕子宫组织的腔上皮或腺上皮中均未检测到酸性FGF免疫染色；然而，观察到了间质染色的差异。在发情周期第10 - 14天从猪收集的子宫组织在整个间质中具有弥漫性aFGF免疫染色。然而，在妊娠早期，随着妊娠进展，强烈的aFGF免疫染色集中在腺上皮周围和腔上皮细胞下方。在围植入期（妊娠第10 - 14天）收集的猪孕体中检测到碱性FGF，但未检测到酸性FGF。尽管酸性和碱性FGF属于同一蛋白质家族，但本研究结果表明它们可能在猪的子宫功能和孕体发育中发挥不同作用。
