Gupta A, Dekaney C M, Bazer F W, Madrigal M M, Jaeger L A
Department of Veterinary Anatomy and Public Health, Institute of Biosciences and Technology, Texas A & M University, College Station, Texas 77843-4458, USA.
Biol Reprod. 1998 Oct;59(4):911-7. doi: 10.1095/biolreprod59.4.911.
Porcine uterine tissues were collected from Days 10 to 14 of gestation (peri-implantation period) or corresponding days of the estrous cycle. Results indicated a marked increase in beta transforming growth factors (TGFbeta1, TGFbeta2, and TGFbeta3) and TGFbeta receptor (type I and type II) immunostaining in uterine luminal epithelium (ULE) between Days 10 and 14 of gestation, but there was no increase in ULE immunostaining on the corresponding days of the estrous cycle. Uterine glands and stroma were intensely immunopositive in pregnant gilts for TGFbeta isoforms and their receptors, but immunostaining was weak to undetectable in cycling gilts. No differences were detected in myometrium, in which immunostaining was moderate in both cycling and pregnant gilts. Additionally, TGFbeta2 and TGFbeta receptor (type I and type II) immunostaining was detected in uterine monocyte/macrophage-like cells. Western blotting detected the presence of all three TGFbeta isoforms in uterine luminal flushings. The CCL64 cell TGFbeta bioassay detected bioactive TGFbetas++ in uterine luminal flushings on Days 12, 13, an 14 of gestation. These results strongly indicate that uterine expression of TGFbetas and their receptors is pregnancy specific and that bioactive TGFbetas are present at the conceptus-maternal interface in the peri-implantation period in pigs. Thus TGFbetas are likely to be involved in autocrine-paracrine interactions between the maternal uterus and the conceptus.
在妊娠第10至14天（着床前期）或发情周期的相应天数收集猪的子宫组织。结果表明，在妊娠第10至14天期间，子宫腔上皮（ULE）中β转化生长因子（TGFβ1、TGFβ2和TGFβ3）和TGFβ受体（I型和II型）的免疫染色显著增加，但在发情周期的相应天数，ULE免疫染色没有增加。妊娠后备母猪的子宫腺和基质对TGFβ异构体及其受体呈强免疫阳性，但在发情周期的后备母猪中免疫染色较弱至无法检测到。在子宫肌层未检测到差异，其中发情周期和妊娠后备母猪的免疫染色均为中等。此外，在子宫单核细胞/巨噬细胞样细胞中检测到TGFβ2和TGFβ受体（I型和II型）免疫染色。蛋白质印迹法检测到子宫腔冲洗液中存在所有三种TGFβ异构体。CCL64细胞TGFβ生物测定法检测到妊娠第12、13和14天子宫腔冲洗液中有生物活性的TGFβs++。这些结果有力地表明，TGFβs及其受体的子宫表达具有妊娠特异性，并且在猪的着床前期，生物活性TGFβs存在于孕体-母体界面。因此，TGFβs可能参与母体子宫与孕体之间的自分泌-旁分泌相互作用。
