Rembrink K, Romijn J C, van der Kwast T H, Rübben H, Schröder F H
Institute of Urology, Erasmus University Rotterdam, The Netherlands.
Prostate. 1997 May 15;31(3):168-74. doi: 10.1002/(sici)1097-0045(19970515)31:3<168::aid-pros4>3.0.co;2-h.
To study the metastatic behavior of human prostate cancer cell lines, orthotopic injection in nude mice was performed.
Local tumor growth, metastasis formation, prostate-specific antigen, and androgen receptor expression were examined.
Hormone-independent cell lines (PC-3, PC-3-125-IL, and TSU-Pr1) were highly tumorigenic and had a higher rate of lymph node metastasis after orthotopic than after subcutaneous implantation. PC-3 cell lines also consistently metastasized to the lungs. The androgen-sensitive LNCaP cell line showed local growth in 7 of 10, and lymph node metastasis in 4 animals. Significant serum PSA levels and strong receptor expression in primary and metastatic tumor tissues were observed.
These results demonstrates that orthotopic implantation of human prostate cancer cell lines, including LNCaP, reproducibly leads to metastasis formation in nude mice.
为研究人前列腺癌细胞系的转移行为，在裸鼠体内进行了原位注射。
检测局部肿瘤生长、转移灶形成、前列腺特异性抗原及雄激素受体表达。
激素非依赖性细胞系（PC-3、PC-3-125-IL和TSU-Pr1）具有高度致瘤性，原位植入后淋巴结转移率高于皮下植入后。PC-3细胞系也始终会转移至肺部。雄激素敏感的LNCaP细胞系在10只动物中有7只出现局部生长，4只出现淋巴结转移。在原发性和转移性肿瘤组织中观察到显著的血清PSA水平及强受体表达。
这些结果表明，包括LNCaP在内的人前列腺癌细胞系原位植入可在裸鼠体内重复性地导致转移灶形成。
