在前列腺癌研究中使用激素敏感型（LNCaP）和激素抵抗型（LNCaP-r）细胞系。
Use of a hormone-sensitive (LNCaP) and a hormone-resistant (LNCaP-r) cell line in prostate cancer research.
作者信息
Pousette A, Carlström K, Henriksson P, Grande M, Stege R
机构信息
Department of Clinical Chemistry, Karolinska Institute, Huddinge University Hospital, Sweden.
出版信息
Prostate. 1997 May 15;31(3):198-203. doi: 10.1002/(sici)1097-0045(19970515)31:3<198::aid-pros9>3.0.co;2-h.
BACKGROUND
In order to develop a hormone-resistant analog to the hormone sensitive cell-line LNCaP, different methods were tried.
METHODS
After almost one year of continuous culture in RPMI-1640, containing a low concentration of androgens, the hormone sensitive cell-line LNCaP became hormone resistant and was named LNCaP-r.
RESULTS
We used the LNCaP/LNCaP-r model system in order to study methods for the parenteral treatment of prostate carcinoma with estrogens, to study the mechanism of action of estramustine, and to identify markers useful as predictive tests for prostate carcinoma.
CONCLUSIONS
A model system made up of the hormone sensitive prostate LNCaP cell-line and the hormone resistant LNCaP-r subline was developed and characterized. This system is, despite a number of limitations, easy to use as a first step to test different hypotheses that can be studied further in later clinical trials.
背景
为了开发一种对激素敏感的细胞系LNCaP的激素抗性类似物，尝试了不同的方法。
方法
在含有低浓度雄激素的RPMI-1640培养基中连续培养近一年后，激素敏感细胞系LNCaP变得对激素具有抗性，并被命名为LNCaP-r。
结果
我们使用LNCaP/LNCaP-r模型系统来研究用雌激素进行前列腺癌胃肠外治疗的方法，研究雌莫司汀的作用机制，并确定可用作前列腺癌预测性检测的标志物。
结论
开发并表征了一个由激素敏感的前列腺LNCaP细胞系和激素抗性LNCaP-r亚系组成的模型系统。尽管存在一些局限性，但该系统易于用作第一步，以测试不同的假设，这些假设可在以后的临床试验中进一步研究。
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