Panek E, Cook G A, Cornell N W
Lipids. 1977 Oct;12(10):814-8. doi: 10.1007/BF02533270.
Fatty acid and cholesterol synthesis in isolated rat hepatocytes were strongly inhibited by 5-(tetradecyloxy)-2-furoic acid. With either 3H2O or [2(-14)C] acetate as the labeled precursor, the concentrations of inhibitor causing 50% decrease in fatty acid and cholesterol synthesis were, respectively, less than 0.005 mM and 0.020 mM. At 0.1 mM inhibitor, citrate concentration in cells from fed rats was increased by 75%; lactate and pyruvate concentrations were decreased by 30%; ethanol oxidation was decreased by 20%; with cells from starved rats, the mitochondrial [NAD+]/[NADH] was decreased. Other parameters were unaffected. Both its potency and its specificity indicate that 5-(tetradecyloxy)-2-furoic acid will be useful in studies on the regulation of lipid biosynthesis.
5-（十四烷氧基）-2-呋喃甲酸能强烈抑制离体大鼠肝细胞中脂肪酸和胆固醇的合成。以3H2O或[2-（14）C]乙酸盐作为标记前体，导致脂肪酸和胆固醇合成减少50%的抑制剂浓度分别低于0.005 mM和0.020 mM。在0.1 mM抑制剂存在下，喂食大鼠的细胞中柠檬酸浓度增加75%；乳酸和丙酮酸浓度降低30%；乙醇氧化降低20%；饥饿大鼠的细胞中，线粒体的[NAD+]/[NADH]降低。其他参数未受影响。5-（十四烷氧基）-2-呋喃甲酸的效力和特异性均表明，它将有助于脂质生物合成调控的研究。
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