Zotikov A A, Polyakov Y S
Microsc Acta. 1977 Sep;79(5):415-8.
The phosphorescence microscope (i.e. a microspectrophosphorimeter) was used for recording the phosphorescence of microobjects. The general optical scheme of the reflecting phosphorescence microscope is described. The recordings of the phosphorescence spectra of cells of various types (bacteria, yeast, protozoa and human fibroblasts) were carried out both at room temperature and deep cooling. It will be shown that the phosphorescence spectra of the microorganisms are specific for every species of cells. The changes of the spectral parameters and triplet decay times after X-irradiation of Tetrahymena pyriformis cells were demonstrated. The decay time of phosphorescence of the fibroblasts at low temperature was also obtained.
磷光显微镜（即显微分光磷光计）用于记录微小物体的磷光。描述了反射式磷光显微镜的一般光学原理。在室温及深度冷却条件下，对各种类型的细胞（细菌、酵母、原生动物和人类成纤维细胞）的磷光光谱进行了记录。结果表明，微生物的磷光光谱对每种细胞类型都具有特异性。证明了梨形四膜虫细胞经X射线照射后光谱参数和三重态衰减时间的变化。还获得了成纤维细胞在低温下的磷光衰减时间。
