Hansen J C, Kreider J I, Demeler B, Fletcher T M
Department of Biochemistry, University of Texas Health Science Center at San Antonio.
Methods. 1997 May;12(1):62-72. doi: 10.1006/meth.1997.0448.
Analytical ultracentrifugation and agarose gel electrophoresis each can be used to accurately quantify changes in structure that accompany chromatin folding in solution. Analytical ultracentrifugation directly measures the extent of compaction of each species present in a chromatin sample under a wide range of solution conditions. Agarose gel electrophoresis yields information about changes in the average surface charge density, size and/or shape, and conformational flexibility during chromatin folding. When used together, these methodologies are particularly powerful. Protocols for the characterization of chromatin folding by analytical ultracentrifugation and agarose gel electrophoresis are described. Discussion focuses on analysis and interpretation of experimental chromatin folding data.
分析超速离心法和琼脂糖凝胶电泳法均可用于准确量化溶液中染色质折叠时伴随的结构变化。分析超速离心法可在广泛的溶液条件下直接测量染色质样品中每种组分的压缩程度。琼脂糖凝胶电泳可提供有关染色质折叠过程中平均表面电荷密度、大小和/或形状以及构象灵活性变化的信息。当这两种方法结合使用时，会特别有效。本文描述了通过分析超速离心法和琼脂糖凝胶电泳法表征染色质折叠的实验方案。讨论重点在于对实验性染色质折叠数据的分析和解读。
