Bellet R E, Danna V, Mastrangelo M J, Berd D
J Natl Cancer Inst. 1979 Nov;63(5):1185-8.
Nine established human melanoma tissue-cultured cell lines heterotransplanted in C57BL/6 "nude" mice were exposed to each of 4 chemotherapeutic agents of known clinical activity against human melanoma. Two of the therapeutic agents, 1,3-bis(2-chloroethyl)-1-nitrosourea (BCNU) and 5-(3,3-dimethyl-1-triazino) imidazole-4-carboxamide (DTIC), are known to be active against human melanoma; the other two, adriamycin and 5-azacytidine, are known to be inactive. Sterile saline served as a control agent. In 2 cell line heterotransplants, the control tumor spontaneously regressed. Of the 7 cell lines that remained for evaluation, 4 were sensitive to DTIC, 1 was sensitive to BCNU, and none was sensitive to adriamycin or 5-azacytidine. These data indicate that the nude mouse-human tumor model may be a predictive secondary screen for cancer chemotherapeutic agents.
将9种已建立的人黑色素瘤组织培养细胞系异种移植到C57BL/6“裸”小鼠体内，使其分别接触4种已知对人黑色素瘤具有临床活性的化疗药物。其中两种治疗药物，1,3-双（2-氯乙基）-1-亚硝基脲（卡莫司汀）和5-（3,3-二甲基-1-三嗪基）咪唑-4-甲酰胺（达卡巴嗪），已知对人黑色素瘤有活性；另外两种，阿霉素和5-氮杂胞苷，已知无活性。无菌生理盐水作为对照剂。在2个细胞系异种移植中，对照肿瘤自发消退。在剩下用于评估的7个细胞系中，4个对达卡巴嗪敏感，1个对卡莫司汀敏感，对阿霉素或5-氮杂胞苷均不敏感。这些数据表明，裸鼠-人肿瘤模型可能是癌症化疗药物的预测性二级筛选模型。
