Katano M, Saxton R E, Cochran A J, Irie R F
J Cancer Res Clin Oncol. 1984;108(2):197-203. doi: 10.1007/BF00402467.
UCLA-SO-M14, a human melanoma cell line, was cultured for ten passages in vitro. The line was converted to an ascitic form, M14-A, by transplanting M14 into CD-1 nude mice and back into tissue culture. The minimum number of M14-A cells that formed ascites in all mice (within 10-21 days) was 5 X 10(5), and over 80% of such mice developed macroscopic liver metastases. M14-A inoculated subcutaneously formed tumor at the site of injection, but rarely led to the development of metastases. However, when M14-A was inoculated subcutaneously in young mice (1-14 days old), more than 50% developed lung (but not liver) metastases. The reproducibility of the formation of ascites and metastases was confirmed by testing M14-A at various passages. M14-A may be useful as a model for the metastatic process in human melanoma.
UCLA-SO-M14是一种人黑色素瘤细胞系，在体外培养了十代。通过将M14移植到CD-1裸鼠体内然后再回到组织培养中，该细胞系转变为腹水形式，即M14-A。在所有小鼠中（10 - 21天内）形成腹水的M14-A细胞的最小数量为5×10⁵，并且超过80%的此类小鼠出现了肉眼可见的肝转移。皮下接种的M14-A在注射部位形成肿瘤，但很少导致转移的发生。然而，当在幼鼠（1 - 14日龄）皮下接种M14-A时，超过50%的幼鼠出现了肺（而非肝）转移。通过在不同传代时测试M14-A，证实了腹水和转移形成的可重复性。M14-A可能作为人类黑色素瘤转移过程的一种模型。
