创伤弧菌血清群E的脂多糖O侧链是鳗鱼的毒力决定因素。
The lipopolysaccharide O side chain of Vibrio vulnificus serogroup E is a virulence determinant for eels.
作者信息
Amaro C, Fouz B, Biosca E G, Marco-Noales E, Collado R
机构信息
Departamento de Microbiología, Facultad de Biología, Universidad de Valencia, Spain.
出版信息
Infect Immun. 1997 Jun;65(6):2475-9. doi: 10.1128/iai.65.6.2475-2479.1997.
Abstract
Vibrio vulnificus is a gram-negative bacterium capable of producing septicemic infections in eels and immunocompromised humans. Two biotypes are classically recognized, with the virulence for eels being specific to strains belonging to biotype 2, which constitutes a homogeneous lipopolysaccharide (LPS)-based O serogroup (which we have designated serogroup E). In the present study we demonstrated that the O side chain of this LPS determines the selective virulence of biotype 2 for eels: (i) biotype 1 strains (which do not belong to serogroup E) are destroyed by the bactericidal action of nonimmune eel serum (NIS) through activation of the alternative pathway of complement, (ii) biotype 2 strains (of serogroup E) are resistant to NIS, and (iii) rough mutants of biotype 2 lacking the O polysaccharide side chain are sensitive to NIS and avirulent for eels.
摘要
创伤弧菌是一种革兰氏阴性菌，能够在鳗鱼和免疫功能低下的人类中引发败血症感染。传统上认可两种生物型，其中对鳗鱼具有毒力的是生物型2的菌株，该生物型构成了一个基于脂多糖（LPS）的同源O血清群（我们将其指定为血清群E）。在本研究中，我们证明了这种LPS的O侧链决定了生物型2对鳗鱼的选择性毒力：（i）生物型1菌株（不属于血清群E）通过补体替代途径的激活被非免疫鳗鱼血清（NIS）的杀菌作用破坏，（ii）生物型2菌株（血清群E）对NIS具有抗性，（iii）缺乏O多糖侧链的生物型2粗糙突变体对NIS敏感且对鳗鱼无毒力。
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