中国仓鼠卵巢细胞次黄嘌呤 - 鸟嘌呤磷酸核糖转移酶位点（CHO/HGPRT系统）突变诱导的定量测定：多种诱变剂的应用

A quantitative assay of mutation induction at the hypoxanthine-guanine phosphoribosyl transferase locus in Chinese hamster ovary cells (CHO/HGPRT system): utilization with a variety of mutagenic agents.

作者信息

O'Neill J P, Couch D B, Machanoff R, San Sebastian J R, Brimer P A, Hsie A W

出版信息

Mutat Res. 1977 Oct;45(1):103-9. doi: 10.1016/0027-5107(77)90048-3.

Abstract

The induction of mutation by a variety of mutagens has been measured utilizing the hypoxanthine-guanine phosphoribosyl transferase (HGPRT) locus in Chinese hamster ovary (CHO) cells (CHO/HGPRT) system). These mutagens include physical agents such as UV light and X-rays, and chemicals such as alkylating agents, ICR-191, and metallic compounds. This system can also be modified for study of the mutagenicity of promutagens such as dimethylnitrosamine (DMN) which require biotransformation for mutagenic action, either through the addition of a rat liver microsomal activation preparation or through a host-mediated activation step using Balb/c athymic mice.

摘要

利用中国仓鼠卵巢（CHO）细胞（CHO/HGPRT）系统中的次黄嘌呤 - 鸟嘌呤磷酸核糖转移酶（HGPRT）基因座，已对多种诱变剂诱导的突变进行了测定。这些诱变剂包括物理因子，如紫外线和X射线，以及化学物质，如烷基化剂、ICR - 191和金属化合物。该系统还可进行修改，用于研究前诱变剂的诱变性，如二甲基亚硝胺（DMN），其诱变作用需要生物转化，可通过添加大鼠肝微粒体激活制剂或通过使用Balb/c无胸腺小鼠的宿主介导激活步骤来实现。