Marklund N, Salci K, Lewén A, Hillered L
Department of Neurosurgery, Uppsala University Hospital, Sweden.
Neuroreport. 1997 Apr 14;8(6):1457-61. doi: 10.1097/00001756-199704140-00026.
Degradation of membrane phospholipids (PLs) is a well known phenomenon in acute brain injuries and is thought to underlie the disturbance of vital cellular membrane functions. In the present study glycerol, an end product of PL degradation, was examined in brain interstitial fluid as a marker of PL breakdown following experimental traumatic brain injury (TBI) using microdialysis. TBI was induced in artificially ventilated rats using the weight-drop technique. The trauma caused a significant, eight-fold increase of dialysate glycerol in the injured cortex, with the peak concentration in the second 10 min fraction after trauma. Glycerol then levelled off but remained significantly above sham-operated controls for the entire 4 h observation period in the perimeter of the injury region where scattered neuronal death is seen. The results support the concept that PL degradation occurs early after TBI and that interstitial glycerol, harvested by microdialysis, may be useful as a marker allowing in vivo monitoring of PL breakdown.
膜磷脂（PLs）的降解是急性脑损伤中一种众所周知的现象，被认为是重要细胞膜功能紊乱的基础。在本研究中，使用微透析技术，对实验性创伤性脑损伤（TBI）后脑间质液中的甘油（PL降解的终产物）进行检测，作为PL分解的标志物。采用重物坠落技术在人工通气的大鼠中诱导TBI。创伤导致损伤皮质中透析液甘油显著增加8倍，创伤后第二个10分钟时段浓度达到峰值。随后甘油水平趋于平稳，但在整个4小时观察期内，在可见散在神经元死亡的损伤区域周边，甘油水平仍显著高于假手术对照组。这些结果支持了TBI后早期发生PL降解的概念，并且通过微透析收集的间质甘油可能作为一种标志物，用于体内监测PL分解。
