Leoncini G, Signorello M G, Piana A, Carrubba M, Armani U
Istituto Policattedra di Chimica Biologica, Università degli Studi di Genova, Italy.
Thromb Res. 1997 Apr 15;86(2):153-60. doi: 10.1016/s0049-3848(97)00058-3.
Free radical activity may contribute to atherosclerotic lesions which in diabetic subjects may frequently lead to vascular complications. It is known that oxidative stress is associated to diabetes. Protein glycation and glucose oxidation could be possible source of free radicals. 28 non insulin dependent diabetic subjects (NIDDM) were examined. 20 healthy subjects matched for age, sex and for the presence of hypertension and hyperlipidemia were also studied. Hydrogen peroxide, measured by intracellular levels of the fluorescent 2,7-dichloro-fluorescein (DCF), was considered as indicative parameter of free radical production. The results showed that in resting platelets the basal level of hydrogen peroxide was significantly higher in diabetic subjects than in controls. Moreover, after stimulation with thrombin, collagen, phorbol myristate acetate (PMA) and platelet activating factor (PAF), platelets of diabetic subjects generated significantly higher amounts of hydrogen peroxide than controls. Moreover, platelet aggregation induced by adenosine 5'-diphosphate (ADP) and plasma beta TG levels were higher in diabetics than in controls. In diabetic patients platelet free radical production and functional activity are increased and therefore could play a role in the elevated thrombotic risk described in diabetes.
自由基活性可能会促使动脉粥样硬化病变的形成，而在糖尿病患者中，这种病变可能经常导致血管并发症。众所周知，氧化应激与糖尿病有关。蛋白质糖基化和葡萄糖氧化可能是自由基的来源。研究人员对28名非胰岛素依赖型糖尿病患者（NIDDM）进行了检查。还研究了20名年龄、性别匹配且患有高血压和高脂血症的健康受试者。通过细胞内荧光2,7-二氯荧光素（DCF）水平测量的过氧化氢被视为自由基产生的指示参数。结果表明，在静息血小板中，糖尿病患者的过氧化氢基础水平显著高于对照组。此外，在用凝血酶、胶原蛋白、佛波酯肉豆蔻酸酯乙酸盐（PMA）和血小板活化因子（PAF）刺激后，糖尿病患者的血小板产生的过氧化氢量显著高于对照组。此外，糖尿病患者中由腺苷5'-二磷酸（ADP）诱导的血小板聚集和血浆β-血小板球蛋白水平高于对照组。在糖尿病患者中，血小板自由基产生和功能活性增加，因此可能在糖尿病中所述的血栓形成风险升高中起作用。
