Madike Lerato N, Pillay M, Popat Ketul C
Department of Biotechnology, Faculty of Applied and Computer Sciences, Vaal University of Technology Andries Potgieter Blvd Vanderbijlpark 1911 South Africa
Department of Mechanical Engineering, School of Biomedical Engineering, Colorado State University Fort Collins Colorado 80523 USA.
RSC Adv. 2021 Sep 13;11(48):30455-30464. doi: 10.1039/d1ra00926e. eCollection 2021 Sep 6.
plant extracts have been investigated for their potential therapeutic effects in the management of various ailments, among which are cardiovascular diseases, due to the wide range of phytocompounds that the plant possesses. One of the major challenges in clinical practice is the inability to control platelet activation and clotting caused by cardiovascular disease interventions. Current treatment methods to inhibit platelet aggregation and thromboxane formation have been associated with major undesirable side effects. This has led to increased research studies on the development of newer and more effective antiplatelet agents. In particular, there has been a growing interest on the potential antiplatelet activity of plant-derived extracts. Hence this study methodically evaluates the anticlotting and antiplatelet properties of aqueous leaf extracts. The platelet activity of the plant extracts was assessed using total platelet adhesion, platelet morphology and whole blood clotting kinetics. The 0.1 mg ml extract mixed with blood plasma demonstrated the lowest platelet adhesion and activation and also reduced whole blood clotting kinetics. There was a reduction of about 70% in platelet adhesion for the 0.1 mg ml treatment compared to the control in the first 15 min which was supported by morphological characterization under SEM. These observations suggest that may be a potential antiplatelet therapeutic agent to inhibit the initial step of platelet adhesion and ultimately reduce the incidence of cardiovascular events.
由于植物含有多种植物化合物，人们对植物提取物在治疗各种疾病（包括心血管疾病）方面的潜在治疗效果进行了研究。临床实践中的一大挑战是无法控制心血管疾病干预导致的血小板激活和凝血。目前抑制血小板聚集和血栓素形成的治疗方法存在严重的不良副作用。这促使人们对开发更新、更有效的抗血小板药物进行了更多研究。特别是，人们对植物提取物的潜在抗血小板活性越来越感兴趣。因此，本研究系统地评估了叶水提取物的抗凝血和抗血小板特性。使用总血小板粘附、血小板形态和全血凝血动力学来评估植物提取物的血小板活性。与血浆混合的0.1毫克/毫升提取物表现出最低的血小板粘附和激活，并且还降低了全血凝血动力学。在最初的15分钟内，与对照组相比，0.1毫克/毫升处理组的血小板粘附减少了约70%，扫描电子显微镜下的形态学特征也证实了这一点。这些观察结果表明，（提取物）可能是一种潜在的抗血小板治疗剂，可抑制血小板粘附的初始步骤，最终降低心血管事件的发生率。
