Romero F J, Zukowski D, Mueller-Klieser W
Institute of Physiology and Pathophysiology, University of Mainz, Germany.
Am J Physiol. 1997 May;272(5 Pt 1):C1507-12. doi: 10.1152/ajpcell.1997.272.5.C1507.
The cellular glutathione (GSH) content of two- and three-dimensional cell cultures of V79 hamster lung cells has been studied. As previously described, cells in monolayer cultures show a decrease in GSH when they reach the confluent state. Three-dimensional cell cultures (multicell spheroids) allow a smoother transition from the initial proliferating to the nonproliferating status, and they show a central area of necrosis when a certain diameter is reached. Cellular GSH content in spheroids is variable throughout the culturing period: 1) GSH content (expressed per mg protein) is lower in spheroids with central necrotic areas than in smaller spheroids without necrosis, and 2) results expressed per cell number show a sharp increase around the diameter where necrosis appears. Once a relatively large necrotic area has been established, GSH decreases again to approximately the prenecrotic level. Interestingly, this GSH "peak" is not dependent on the time in culture but on the spheroid size. Acute hypoxia occurs in central areas of spheroids at a much higher size range than those described herein. Thus we suggest a combination of factors, which may include oxidative stress among others, as the explanation for these cellular GSH variations.
对V79仓鼠肺细胞的二维和三维细胞培养物中的细胞内谷胱甘肽（GSH）含量进行了研究。如前所述，单层培养的细胞在达到汇合状态时GSH含量会降低。三维细胞培养物（多细胞球体）允许从初始增殖状态到非增殖状态的更平稳过渡，并且当达到一定直径时会显示出中央坏死区域。在整个培养期间，球体中的细胞GSH含量是可变的：1）具有中央坏死区域的球体中GSH含量（以每毫克蛋白质表示）低于没有坏死的较小球体；2）以每个细胞数表示的结果显示，在出现坏死的直径附近急剧增加。一旦建立了相对较大的坏死区域，GSH又会下降到大约坏死前的水平。有趣的是，这种GSH“峰值”不取决于培养时间，而是取决于球体大小。球体中央区域发生急性缺氧的大小范围比本文所述的要高得多。因此，我们提出多种因素的组合，其中可能包括氧化应激等，作为这些细胞GSH变化的解释。
