Onuki Y, Hayashida N, Wu M H, Shi Q, Kouchi Y, Ghali R, Sauvage L R
Department of Surgery, University of Washington School of Medicine, Seattle, USA.
Ann Vasc Surg. 1997 Mar;11(2):141-8. doi: 10.1007/s100169900024.
Accelerated endothelialization was studied by creating a vascular tissue environment around porous Dacron grafts. Three study groups, each containing 10 dogs, were divided equally into 2- and 4-week implant periods, with 8 mm x 6 cm knitted Dacron grafts implanted in the abdominal aorta. Grafts in group 1, the control group, were implanted conventionally. In group 2 each implanted graft was completely wrapped in a resected segment of the autogenous inferior vena cava, with its intima against the wall. In group 3 the adventitial side of the vein was wrapped against the wall. The vein wrap produced accelerated endothelialization as follows: endothelial-like cell coverage scores at 2 and 4 weeks were, respectively, 78% and 98% for group 2 and 80% and 95% for group 3, compared to only 14% and 50% for group 1 (p < 0.05). The neointima, which contained smooth muscle cells, was formed as early as 2 weeks in the vein-wrapped grafts. There were no differences in the speed of healing or in healing patterns according to whether the intimal or the adventitial side of the inferior vena cava was placed against the graft. Histologic findings did not support the hypothesis that accelerated flow surface endothelialization results in direct migration of endothelial cells from the intima of the vein wrap, and there was no clear correlation between the surface endothelial-like cell coverage and microostia. To gain further insight into why accelerated healing occurs in this model, earlier observations accompanied by molecular biology analysis are needed, and vein wrap studies provide a method of comparison for this work.
通过在多孔涤纶移植物周围创建血管组织环境来研究加速内皮化。三个研究组，每组包含10只狗，被平均分为2周和4周的植入期，将8mm×6cm的针织涤纶移植物植入腹主动脉。第1组为对照组，移植物按常规方式植入。第2组中，每个植入的移植物完全包裹在自体下腔静脉的切除段中，其内膜靠壁。第3组中，静脉的外膜侧靠壁包裹。静脉包裹产生了加速内皮化，如下所示：第2组在2周和4周时内皮样细胞覆盖分数分别为78%和98%，第3组为80%和95%，而第1组仅为14%和50%（p<0.05）。在静脉包裹的移植物中，早在2周时就形成了含有平滑肌细胞的新生内膜。根据下腔静脉的内膜或外膜侧是否靠在移植物上，愈合速度或愈合模式没有差异。组织学结果不支持加速血流表面内皮化导致内皮细胞从静脉包裹内膜直接迁移的假说，并且表面内皮样细胞覆盖与微孔之间没有明显的相关性。为了进一步深入了解该模型中加速愈合发生的原因，需要早期观察并进行分子生物学分析，而静脉包裹研究为此项工作提供了一种比较方法。
