Varga Z, Vajtai I, Aguzzi A
Institute of Clinical Pathology, University Hospital Zürich, Switzerland.
Pathol Res Pract. 1996 Dec;192(12):1225-31. doi: 10.1016/s0344-0338(96)80155-5.
Isoforms of the CD44 adhesion molecule have been assigned a pivotal role in tumor invasion and metastasis. CD44 splice variants may be selectively expressed in various normal and neoplastic tissues. We investigated immunohistochemically the presence of the standard (H) and two variant (v3, v6) isoforms of the CD44 molecule in a series comprising 13 choroid plexus papillomas (WHO grade I) and 8 carcinomas (WHO grade III). In the papilloma group, 5 tumors showed variable cellular pleomorphism and foci of infiltrative growth, and were tentatively classified as atypical papillomas. Autopsy specimens of normal pediatric and adult choroid plexus were used as control. Western-blot analysis of CD44H was carried out on 4 carcinomas, 1 papilloma and on normal choroid plexus. The proliferation rate was assessed by MIB-1 immunoreactivity. The normal choroid plexuses and 9 papillomas were negative for the standard as well as the variant CD44 molecules investigated. Four atypical papillomas and 5 carcinomas expressed CD44H. CD44v3 and CD44v6 were only detected in one of the atypical papillomas also positive for CD44H. These data indicate that CD44H is preferentially expressed on atypical papillomas and carcinomas and may correlate with the infiltrative growth of these tumors.
CD44黏附分子的亚型在肿瘤侵袭和转移中发挥着关键作用。CD44剪接变体可能在各种正常组织和肿瘤组织中选择性表达。我们采用免疫组织化学方法，对13例脉络丛乳头状瘤（WHO I级）和8例癌（WHO III级）组成的系列病例中CD44分子的标准型（H）和两种变异型（v3、v6）亚型的存在情况进行了研究。在乳头状瘤组中，5例肿瘤表现出不同程度的细胞多形性和浸润性生长灶，暂被归类为非典型乳头状瘤。正常儿童和成人脉络丛的尸检标本用作对照。对4例癌、1例乳头状瘤和正常脉络丛进行了CD44H的蛋白质印迹分析。通过MIB-1免疫反应性评估增殖率。所研究的正常脉络丛和9例乳头状瘤的标准型及变异型CD44分子均为阴性。4例非典型乳头状瘤和5例癌表达CD44H。CD44v3和CD44v6仅在1例CD44H也呈阳性的非典型乳头状瘤中检测到。这些数据表明，CD44H在非典型乳头状瘤和癌中优先表达，可能与这些肿瘤的浸润性生长相关。
