Glansbeek H L, van Beuningen H M, Vitters E L, Morris E A, van der Kraan P M, van den Berg W B
University Hospital Nijmegen, The Netherlands.
Arthritis Rheum. 1997 Jun;40(6):1020-8. doi: 10.1002/art.1780400605.
To study the effect of bone morphogenetic protein 2 (BMP-2) on articular cartilage proteoglycan (PG) synthesis in vivo and to investigate whether BMP-2 is able to counteract the effects of interleukin-1 (IL-1) on articular cartilage PG synthesis and content.
BMP-2 alone or in combination with IL-1alpha was injected into murine knee joints. PG synthesis was measured by 35S-sulfate incorporation using an ex vivo method or autoradiography. Cartilage PG content was analyzed by measuring Safranin O staining intensity on histologic sections.
BMP-2 appeared to be a potent stimulator of articular cartilage PG synthesis in vivo. However, BMP-2 was not able to counteract the deleterious effects of IL-1alpha on articular cartilage PG synthesis and content. In addition, intraarticular injections of BMP-2 induced chondrophytes.
Although BMP-2 is a very potent stimulator of cartilage PG synthesis in vivo, the therapeutic applications of BMP-2 are limited due to the inability of BMP-2 to counteract the effects of IL-1 and the induction of chondrophytes.
研究骨形态发生蛋白2（BMP - 2）对体内关节软骨蛋白聚糖（PG）合成的影响，并探讨BMP - 2是否能够抵消白细胞介素 - 1（IL - 1）对关节软骨PG合成及含量的影响。
将BMP - 2单独或与IL - 1α联合注射到小鼠膝关节内。采用体外方法或放射自显影通过35S - 硫酸盐掺入法测量PG合成。通过测量组织学切片上番红O染色强度分析软骨PG含量。
BMP - 2似乎是体内关节软骨PG合成的有效刺激剂。然而，BMP - 2无法抵消IL - 1α对关节软骨PG合成及含量的有害影响。此外，关节内注射BMP - 2可诱导软骨细胞。
尽管BMP - 2在体内是软骨PG合成的非常有效的刺激剂，但由于BMP - 2无法抵消IL - 1的影响以及诱导软骨细胞，其治疗应用受到限制。
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