Bourguignon J P, Dourcy C, Franchimont P
C R Seances Soc Biol Fil. 1976;170(1):194-9.
A method of extraction of synthetic LHRH is studied in human urines, using porous glass (Spherosil) and methanol. In the defined conditions the yield is greeter than 80%. It appears that the method is reproducible. The recovery varies essentially with the quantity of Spherosil and the pH of methanal. The use of methanol acidified at pH 3 increases the speed and the importance of labelled LHRH recovery.
研究了一种使用多孔玻璃(Spherosil)和甲醇从人尿中提取合成促黄体生成素释放激素(LHRH)的方法。在规定条件下,产率大于80%。该方法似乎具有可重复性。回收率主要随Spherosil的用量和甲醛的pH值而变化。使用pH值为3的酸化甲醇可提高标记LHRH回收的速度和回收率。