Laham N, Van Dunné F, Abraham L J, Farrugia W, Bendtzen K, Brennecke S P, Rice G E
Department of Perinatal Medicine, Royal Women's Hospital, Carlton, Victoria, Australia.
J Reprod Immunol. 1997 Apr;33(1):53-69. doi: 10.1016/s0165-0378(97)01012-7.
The aims of this study were to determine tumor necrosis factor-beta (TNF-beta) concentration profiles in peripheral venous plasma and amniotic fluid during pregnancy and at the time of labor and to characterise TNF-beta mRNA expression and TNF-beta release from human gestational tissues. In addition, we investigated the expression of TNF-beta binding protein, lymphotoxin-beta (LT-beta), in human gestational tissues. The mean (+/-S.E.M.) TNF-beta concentrations in maternal plasma (TIL, 78 +/- 12 pg/ml, n = 7 vs. TNIL, 304 +/- 88 pg/ml, n = 7) and amniotic fluid (TIL, 8 +/- 5 pg/ml, n = 6 vs. TNIL, 73 +/- 20 pg/ml, n = 20) were significantly (P < 0.05) decreased in association with term labor-onset (TIL) compared to term not-in-labor (TNIL). TNF-beta concentration in maternal plasma and amniotic fluid did not change significantly either with preterm labor (PIL), or during pregnancy. Group-matched comparison of maternal plasma and amniotic fluid TNF-beta concentrations demonstrated that amniotic fluid TNF-beta concentrations were 6-8 fold lower than maternal plasma TNF-beta concentrations. Furthermore, no detectable TNF-beta was secreted from cultured human amniotic, choriodecidual and placental explants. Although, TNF-beta mRNA was detected in amnion, choriodecidual and placenta, LT-beta was similarly expressed in these tissues, suggesting that TNF-beta may be cell membrane bound. These data demonstrate that TNF-beta is present at low levels within the intrauterine environment and may suggest that TNF-beta is specifically inhibited at the maternal-fetal interface.
本研究的目的是测定孕期及分娩时外周静脉血浆和羊水中肿瘤坏死因子-β（TNF-β）的浓度变化，并对人妊娠组织中TNF-β mRNA表达及TNF-β释放进行特征分析。此外，我们还研究了人妊娠组织中TNF-β结合蛋白淋巴毒素-β（LT-β）的表达情况。与未临产足月（TNIL）相比，临产足月（TIL）时母体血浆（TIL，78±12 pg/ml，n = 7 vs. TNIL，304±88 pg/ml，n = 7）和羊水（TIL，8±5 pg/ml，n = 6 vs. TNIL，73±20 pg/ml，n = 20）中TNF-β的平均（±标准误）浓度显著降低（P < 0.05）。母体血浆和羊水中的TNF-β浓度在早产（PIL）时或孕期均无显著变化。母体血浆和羊水TNF-β浓度的组间匹配比较显示，羊水TNF-β浓度比母体血浆TNF-β浓度低6 - 8倍。此外，培养的人羊膜、绒毛膜蜕膜和胎盘外植体未分泌可检测到的TNF-β。虽然在羊膜、绒毛膜蜕膜和胎盘中检测到了TNF-β mRNA，但LT-β在这些组织中也有类似表达，提示TNF-β可能与细胞膜结合。这些数据表明，TNF-β在子宫内环境中含量较低，可能提示TNF-β在母胎界面受到特异性抑制。
