Laham N, Brennecke S P, Bendtzen K, Rice G E
Department of Perinatal Medicine, Royal Women's Hospital, Victoria, Australia.
J Endocrinol. 1996 Jun;149(3):431-9. doi: 10.1677/joe.0.1490431.
In this study, we quantified interleukin-6 (IL-6) concentrations in amniotic fluid at term and preterm labour, and determined the gestational tissue source of IL-6. In addition, aspects of the regulatory mechanisms involved in IL-6 release at the time of term labour and in response to bacterial endotoxin, lipopolysaccharide (LPS), have been established. IL-6 concentrations were 2-fold higher in amniotic fluid collected at term compared with preterm gestation, with an additional 2-fold increase in association with term labour. IL-6 was released from all choriodecidual and placental explants but was detected in only 33% of amniotic explant cultures of tissues obtained before labour onset. In contrast, IL-6 was detected in all amniotic, choriodecidual and placental cultures of tissues obtained after term labour onset and delivery, and the mean IL-6 release was significantly higher than that measured in explant cultures of both amniotic (80-fold increase, P < 0.0001) and choriodecidual (3-fold increase, P < 0.02) but not placental explants taken at the time of elective Caesarean section at term before labour onset. LPS significantly (P < 0.05) increased the release of IL-6 from human choriodecidual and placental explants but not amniotic explants, in a time- and dose-dependent manner. IL-6 is a physiological constituent of amniotic fluid and its production by gestational tissues is differentially regulated by LPS and spontaneous labour onset and delivery.
在本研究中，我们对足月分娩和早产时羊水白细胞介素-6（IL-6）浓度进行了定量，并确定了IL-6的妊娠组织来源。此外，还确定了足月分娩时以及对细菌内毒素脂多糖（LPS）反应时参与IL-6释放的调节机制。与早产相比，足月收集的羊水中IL-6浓度高2倍，且与足月分娩相关时会额外增加2倍。所有绒毛蜕膜和胎盘外植体均释放IL-6，但在分娩发动前获取的组织的羊膜外植体培养物中仅33%检测到IL-6。相反，在足月分娩发动和分娩后获取的组织的所有羊膜、绒毛蜕膜和胎盘培养物中均检测到IL-6，且IL-6平均释放量显著高于足月分娩发动前择期剖宫产时获取的羊膜（增加80倍，P<0.0001）和绒毛蜕膜（增加3倍，P<0.02）外植体培养物中的测量值，但胎盘外植体除外。LPS以时间和剂量依赖性方式显著（P<0.05）增加人绒毛蜕膜和胎盘外植体中IL-6的释放，但不增加羊膜外植体中IL-6的释放。IL-6是羊水的一种生理成分，其由妊娠组织产生受到LPS、自然分娩发动和分娩的差异调节。
