Wagner H, Baretton G B, Schneiderbanger K, Nerlich A, Bise K, Löhrs U
Institute of Pathology, University of Munich, Germany.
Pediatr Pathol Lab Med. 1997 May-Jun;17(3):401-12.
Teratomas are neoplasms that are composed of tissues from all three germinal layers. The exact histogenetic origin of teratomas, however, is still controversial. In order to gain more insight into histogenesis of extragonadal teratomas (EGTs), the gonosomal status in 13 congenital EGTs was studied by means of interphase cytogenetics using nonradioactive in situ hybridization (NISH) with centromere-specific DNA probes. By use of this technique a direct correlation of cytogenetic results with morphology was possible. In all EGTs analyzed the gonosomal status in tissues derived from the different germinal layers was identical to that of the nontumorous fetal and placental tissue. This was true irrespective of localization, age, histological type, and classification of the EGT. Our results strongly suggest that EGTs arise from pluripotent diploid precursor cells, for example, either premeiotic germ cells that have not yet undergone the first meiotic division or pluripotent ectopic embryonal or extraembryonal cells. Our data do not support the theory of parthenogenetic EGT development, at least in males.
畸胎瘤是由来自所有三个胚层的组织构成的肿瘤。然而，畸胎瘤的确切组织发生起源仍存在争议。为了更深入了解性腺外畸胎瘤（EGT）的组织发生，我们采用非放射性原位杂交（NISH）技术，使用着丝粒特异性DNA探针，通过间期细胞遗传学方法研究了13例先天性EGT的性染色体状态。利用该技术可以将细胞遗传学结果与形态学直接关联起来。在所有分析的EGT中，来自不同胚层的组织中的性染色体状态与非肿瘤性胎儿和胎盘组织的性染色体状态相同。无论EGT的定位、年龄、组织学类型和分类如何，都是如此。我们的结果强烈表明，EGT起源于多能二倍体前体细胞，例如，尚未经历第一次减数分裂的减数分裂前生殖细胞或多能异位胚胎或胚外细胞。我们的数据不支持孤雌生殖性EGT发育的理论，至少在男性中是这样。
