通过端粒重复序列扩增法（TRAP）对端粒酶活性进行定量和表征的进展。
Advances in quantification and characterization of telomerase activity by the telomeric repeat amplification protocol (TRAP).
作者信息
Kim N W, Wu F
机构信息
Geron Corporation, Menlo Park, CA 94025, USA.
出版信息
Nucleic Acids Res. 1997 Jul 1;25(13):2595-7. doi: 10.1093/nar/25.13.2595.
Abstract
The telomeric repeat amplification protocol (TRAP) assay has been used to test telomerase activity in numerous cancer specimens. We describe primers, controls and quantification methods for the TRAP assay to accurately measure the level of telomerase activity in clinical samples. The assay is reliable and reproducible in routine analyses and can be used to estimate the processivity of telomerase activity.
摘要
端粒重复序列扩增法（TRAP）检测已被用于检测众多癌症标本中的端粒酶活性。我们描述了TRAP检测的引物、对照和定量方法，以准确测量临床样本中端粒酶活性水平。该检测在常规分析中可靠且可重复，可用于评估端粒酶活性的持续合成能力。
相似文献
1
Advances in quantification and characterization of telomerase activity by the telomeric repeat amplification protocol (TRAP).通过端粒重复序列扩增法（TRAP）对端粒酶活性进行定量和表征的进展。
Nucleic Acids Res. 1997 Jul 1;25(13):2595-7. doi: 10.1093/nar/25.13.2595.
2
Telomeric repeat amplification, without shortening or lengthening of the telomerase products: a method to analyze the processivity of telomerase enzyme.端粒重复序列扩增，端粒酶产物无缩短或延长：一种分析端粒酶酶持续合成能力的方法。
Nucleic Acids Res. 2001 Jan 15;29(2):E3. doi: 10.1093/nar/29.2.e3.
3
Detection of telomerase activity utilizing energy transfer primers: comparison with gel- and ELISA-based detection.利用能量转移引物检测端粒酶活性：与基于凝胶和酶联免疫吸附测定的检测方法的比较
Biotechniques. 1999 Mar;26(3):552-8. doi: 10.2144/99263pf01.
4
Assessment and quantification of telomerase enzyme activity.端粒酶活性的评估与定量
Methods Mol Biol. 2013;965:215-31. doi: 10.1007/978-1-62703-239-1_14.
5
SYBR Green real-time telomeric repeat amplification protocol for the rapid quantification of telomerase activity.用于快速定量端粒酶活性的SYBR Green实时端粒重复序列扩增方案。
Nucleic Acids Res. 2003 Jan 15;31(2):E3-3. doi: 10.1093/nar/gng003.
6
Analysis of telomerase activity and telomere function in cancer.癌症中端粒酶活性与端粒功能的分析
Methods Mol Biol. 2004;281:333-48. doi: 10.1385/1-59259-811-0:333.
7
[Development of in situ TRAP assay detecting telomerase activity in cell].[用于检测细胞中端粒酶活性的原位TRAP分析方法的开发]
Nihon Rinsho. 1998 May;56(5):1159-64.
8
9
Nonradioactive detection of telomerase activity using the telomeric repeat amplification protocol.使用端粒重复序列扩增法对端粒酶活性进行非放射性检测。
Nat Protoc. 2006;1(3):1583-90. doi: 10.1038/nprot.2006.239.
10
A quantitative method to measure telomerase activity by bioluminescence connected with telomeric repeat amplification protocol.一种通过生物发光结合端粒重复序列扩增协议来测量端粒酶活性的定量方法。
Anal Biochem. 2001 Dec 15;299(2):188-93. doi: 10.1006/abio.2001.5418.
引用本文的文献
1
Broad-spectrum antitumor analysis of the telomerase activity inhibitor TPCH derived from the human constitutively expressed protein LPTS/PinX1.源自人类组成性表达蛋白LPTS/PinX1的端粒酶活性抑制剂TPCH的广谱抗肿瘤分析
Front Oncol. 2025 Aug 15;15:1643155. doi: 10.3389/fonc.2025.1643155. eCollection 2025.
2
NONO, SFPQ, and PSPC1 promote telomerase recruitment to the telomere.NONO、SFPQ和PSPC1促进端粒酶募集至端粒。
Nat Commun. 2025 Jul 1;16(1):5769. doi: 10.1038/s41467-025-60924-w.
3
High-Throughput Screening Tool to Identify Small Molecule Inhibitors of Telomerase.用于鉴定端粒酶小分子抑制剂的高通量筛选工具
ACS Chem Biol. 2025 Jul 18;20(7):1707-1714. doi: 10.1021/acschembio.5c00244. Epub 2025 Jun 10.
4
Leukocyte telomere length change in children with obesity in the context of an isocaloric fructose restriction intervention.在等热量果糖限制干预背景下肥胖儿童的白细胞端粒长度变化
Diabetol Metab Syndr. 2025 Mar 22;17(1):94. doi: 10.1186/s13098-025-01611-0.
5
Telomeres and telomerase in mesothelioma: Pathophysiology, biomarkers and emerging therapeutic strategies (Review).间皮瘤中的端粒与端粒酶：病理生理学、生物标志物及新兴治疗策略（综述）
Int J Oncol. 2025 Mar;66(3). doi: 10.3892/ijo.2025.5729. Epub 2025 Feb 21.
6
Preparation and anticancer activity of telomerase inhibitor TAT-LPTS39 polypeptide.端粒酶抑制剂TAT-LPTS39多肽的制备及其抗癌活性
Transl Cancer Res. 2024 Sep 30;13(9):4625-4638. doi: 10.21037/tcr-24-792. Epub 2024 Sep 27.
7
mTert induction in p21-positive cells counteracts capillary rarefaction and pulmonary emphysema.p21阳性细胞中的mTert诱导可对抗毛细血管稀疏和肺气肿。
EMBO Rep. 2024 Mar;25(3):1650-1684. doi: 10.1038/s44319-023-00041-1. Epub 2024 Feb 29.
8
Thermal Preconditioning Alters the Stability of Hump-Snout Whitefish () and Its Hybrid Form, Showing Potential for Aquaculture.热预处理改变驼背白鲑及其杂交形态的稳定性，显示出其在水产养殖中的潜力。
Biology (Basel). 2023 Oct 20;12(10):1348. doi: 10.3390/biology12101348.
9
Imaging telomerase reverse transcriptase expression in oligodendrogliomas using hyperpolarized δ-[1-C]-gluconolactone.使用超极化δ-[1-C]-葡萄糖酸内酯成像少突胶质细胞瘤中端粒酶逆转录酶的表达。
Neurooncol Adv. 2023 Jul 21;5(1):vdad092. doi: 10.1093/noajnl/vdad092. eCollection 2023 Jan-Dec.
10
Novel pathological variants of NHP2 affect N-terminal domain flexibility, protein stability, H/ACA Ribonucleoprotein (RNP) complex formation and telomerase activity.新型 NHP2 病理性变异影响 N 端结构域的灵活性、蛋白稳定性、H/ACA 核糖核蛋白（RNP）复合物形成和端粒酶活性。
Hum Mol Genet. 2023 Sep 16;32(19):2901-2912. doi: 10.1093/hmg/ddad114.
本文引用的文献
1
Specific association of human telomerase activity with immortal cells and cancer.人类端粒酶活性与永生化细胞及癌症的特定关联。
Science. 1994 Dec 23;266(5193):2011-5. doi: 10.1126/science.7605428.
2
Telomerase, cell immortality, and cancer.端粒酶、细胞永生化与癌症。
Cold Spring Harb Symp Quant Biol. 1994;59:307-15. doi: 10.1101/sqb.1994.059.01.035.
3
Correlating telomerase activity levels with human neuroblastoma outcomes.将端粒酶活性水平与人类神经母细胞瘤的预后相关联。
Nat Med. 1995 Mar;1(3):249-55. doi: 10.1038/nm0395-249.
4
Modifications of a telomeric repeat amplification protocol (TRAP) result in increased reliability, linearity and sensitivity.端粒重复序列扩增 protocol (TRAP) 的改进提高了可靠性、线性度和灵敏度。
Nucleic Acids Res. 1995 Sep 25;23(18):3794-5. doi: 10.1093/nar/23.18.3794.
5
Identification of a specific telomere terminal transferase activity in Tetrahymena extracts.在四膜虫提取物中鉴定出一种特定的端粒末端转移酶活性。
Cell. 1985 Dec;43(2 Pt 1):405-13. doi: 10.1016/0092-8674(85)90170-9.
Zotero 插件