Ohshima K, Suzumiya J, Ohga S, Ohgami A, Kikuchi M
Department of Pathology, School of Medicine, Fukuoka University, Jonan-ku, Japan.
Int J Cancer. 1997 Jun 11;71(6):943-7. doi: 10.1002/(sici)1097-0215(19970611)71:6<943::aid-ijc5>3.0.co;2-y.
In order to examine the role of Epstein-Barr virus (EBV) in the pathogenesis of chronic active EBV infection (CAEBV), we investigated whether or not EBV integration into the human genome is associated with any chromosonal abnormality. We therefore analyzed 4 cases of CAEBV: 2 cases showed a normal karyotype, while one had an oligo-clonal 6th chromosomal abnormality and the fourth had a clonal 6th deletion (q15q23). In addition, the case with an oligo-clonal abnormality also had oligo-clonal EBV terminal repeat (TR) bands, while the case with a clonal abnormality showed a clonal TR band. In contrast, the 2 cases with a normal karyotype showed no clonal band. Two-color fluorescence in situ hybridization (FISH) was used to detect the integrated EBV and the 6th chromosomal site. The presence of integrated EBV into the 6th chromosome was not frequent in the 2 cases with a normal karyotype, but it was statistically frequent in the case with an oligo-clonal 6th abnormality. In the case with a clonal 6th deletion, integration in the 6th chromosome was also slightly higher than that in the other chromosomes. In CAEBV, integrated EBV might thus be associated both with chromosomal abnormality and with pathogenesis.
为了研究爱泼斯坦-巴尔病毒（EBV）在慢性活动性EBV感染（CAEBV）发病机制中的作用，我们调查了EBV整合入人类基因组是否与任何染色体异常有关。因此，我们分析了4例CAEBV：2例核型正常，1例有寡克隆性6号染色体异常，第4例有克隆性6号染色体缺失（q15q23）。此外，有寡克隆性异常的病例也有寡克隆性EBV末端重复序列（TR）条带，而有克隆性异常的病例显示有克隆性TR条带。相比之下，2例核型正常的病例未显示克隆条带。采用双色荧光原位杂交（FISH）检测整合的EBV和6号染色体位点。在2例核型正常的病例中，EBV整合到6号染色体的情况并不常见，但在有寡克隆性6号染色体异常的病例中在统计学上较为常见。在有克隆性6号染色体缺失的病例中，6号染色体上的整合也略高于其他染色体。因此，在CAEBV中，整合的EBV可能与染色体异常和发病机制均有关。
