Ohshima K, Suzumiya J, Kanda M, Kato A, Kikuchi M
Department of Pathology, School of Medicine, Fukuoka University, Japan.
Cancer Lett. 1998 Jan 9;122(1-2):43-50. doi: 10.1016/s0304-3835(97)00368-6.
Epstein-Barr virus (EBV) is known to be linear in viral particles but EBV circularizes into an episomal form after infection. Recently, the presence of integrated EBV DNA has been reported. We investigated EBV integration into the human genome in EBV-associated disease using Southern blotting. One hundred four cases in which the presence of EBV was confirmed by Southern blotting with EBV-W probes were thus analyzed with left- and right-hand end probes of linear EBV. Integrated EBV was demonstrated in 11 of 104 cases; five of 14 cases with B cell lymphoma (36%), one of 12 cases with nasopharyngeal carcinomas (8%), four of 31 cases with natural killer (NK) leukemia/lymphoma (13%) and one of 11 cases with chronic EBV infection (9%). However, none of the 24 T cell lymphoma, seven Hodgkin's disease, or five acute EBV infection cases showed integrated EBV. In addition, seven of the 11 cases with EBV integration (five B cell lymphoma and two NK leukemia/lymphoma) showed only an integration form, however, the other four (two NK leukemia/lymphoma, one nasopharyngeal carcinoma and one chronic EBV infection) showed both integrated and episomal forms. The integrated form was frequently found in B cell lymphoma and especially in high grade B cell lymphoma. Fluorescence in situ hybridization (FISH) was performed in two cases (NK and B cell lymphoma), which represented integrated EBV in Southern blotting and the integration form was confirmed in both. However, it is still uncertain as to whether or not the EBV integration site is directly associated with chromosomal abnormality.
已知爱泼斯坦-巴尔病毒（EBV）在病毒颗粒中呈线性，但感染后EBV会环化形成附加体形式。最近，有报道称存在整合的EBV DNA。我们使用Southern印迹法研究了EBV在EBV相关疾病中整合到人类基因组的情况。因此，对104例经EBV-W探针Southern印迹法证实存在EBV的病例，用线性EBV的左手端和右手端探针进行了分析。在104例病例中有11例显示存在整合的EBV；14例B细胞淋巴瘤中有5例（36%），12例鼻咽癌中有1例（8%），31例自然杀伤（NK）白血病/淋巴瘤中有4例（13%），11例慢性EBV感染中有1例（9%）。然而，24例T细胞淋巴瘤、7例霍奇金病或5例急性EBV感染病例中均未显示整合的EBV。此外，11例有EBV整合的病例中有7例（5例B细胞淋巴瘤和2例NK白血病/淋巴瘤）仅显示整合形式，然而，其他4例（2例NK白血病/淋巴瘤、1例鼻咽癌和1例慢性EBV感染）显示整合形式和附加体形式均有。整合形式在B细胞淋巴瘤中经常发现，尤其是在高级别B细胞淋巴瘤中。对2例病例（NK和B细胞淋巴瘤）进行了荧光原位杂交（FISH），这2例在Southern印迹法中代表整合的EBV，并且在两者中均证实了整合形式。然而，EBV整合位点是否与染色体异常直接相关仍不确定。
