Pérez-Ruiz T, Martínez-Lozano C, Sanz A, Bravo E
Department of Analytical Chemistry, Faculty of Chemistry, University of Murcia, Spain.
J Chromatogr B Biomed Sci Appl. 1997 Apr 25;692(1):199-205. doi: 10.1016/s0378-4347(96)00482-3.
Data presented in this paper show that bromhexine and its pharmacologically active metabolite can easily be determined by capillary zone electrophoresis. The composition of the running buffer had a significant effect on the reproducibility of the migration time for which a carrier solution containing 30 mM phosphate buffer (pH 3.0), 5 M urea and 10% (v/v) acetonitrile was used. The method was validated with respect to its response linearity and reproducibility. The method is suitable for the determination of bromhexine and ambroxol in several samples such as pharmaceuticals, urine and serum. Photodiode-array detection permitted the rapid identification of both drugs in the sample analyzed.
本文所呈现的数据表明，氨溴索及其药理活性代谢物可通过毛细管区带电泳轻松测定。运行缓冲液的组成对迁移时间的重现性有显著影响，为此使用了含有30 mM磷酸盐缓冲液（pH 3.0）、5 M尿素和10%（v/v）乙腈的载体溶液。该方法在响应线性和重现性方面得到了验证。该方法适用于测定多种样品中的氨溴索和氨溴索，如药物、尿液和血清。光电二极管阵列检测可快速鉴定分析样品中的两种药物。
