Ulmasov T, Hagen G, Guilfoyle T J
Department of Biochemistry, University of Missouri, 117 Schweitzer Hall, Columbia, MO 65211, USA.
Science. 1997 Jun 20;276(5320):1865-8. doi: 10.1126/science.276.5320.1865.
The plant hormone auxin regulates plant physiology by modulating the interaction of transcription factors with auxin response elements (AuxREs) of the affected genes. A transcription factor, Auxin Response Factor 1 (ARF1), that binds to the sequence TGTCTC in AuxREs was cloned from Arabidopsis by using a yeast one-hybrid system. ARF1 has an amino-terminal DNA-binding domain related to the carboxyl terminus of the maize transactivator Viviparous-1. Sequence requirements for ARF1 binding in vitro are identical to those that confer auxin responsiveness in vivo. The carboxyl terminus of ARF1 contains two motifs found in the Aux/IAA class of proteins and appears to mediate protein-protein interactions.
植物激素生长素通过调节转录因子与受影响基因的生长素反应元件（AuxREs）之间的相互作用来调控植物生理。利用酵母单杂交系统从拟南芥中克隆出一种与AuxREs中TGTCTC序列结合的转录因子——生长素反应因子1（ARF1）。ARF1具有一个与玉米反式激活因子Viviparous-1羧基末端相关的氨基末端DNA结合结构域。ARF1体外结合的序列要求与体内赋予生长素反应性的序列要求相同。ARF1的羧基末端包含在Aux/IAA类蛋白质中发现的两个基序，似乎介导蛋白质-蛋白质相互作用。
