Romanowski M J, Garrido-Guerrero E, Shenk T
Department of Molecular Biology, Howard Hughes Medical Institute, Princeton University, New Jersey 08544-1014, USA.
J Virol. 1997 Jul;71(7):5703-5. doi: 10.1128/JVI.71.7.5703-5705.1997.
The virus-coded proteins pIRS1 and pTRS1 were found associated with purified human cytomegalovirus virions. The proteins were not degraded when intact virions were treated with trypsin, which suggests that they are localized inside the viral particle. In transfection experiments pIRS1 and pTRS1 modestly activated expression from a reporter plasmid containing the viral major immediate-early promoter but did not influence the activity of a reporter carrying the irs1/trs1 immediate-early promoter. Both reporters were activated by the combination of pIRS1 or pTRS1 and pUL69, which is also present in virions.
发现病毒编码蛋白pIRS1和pTRS1与纯化的人巨细胞病毒病毒粒子相关。当完整的病毒粒子用胰蛋白酶处理时，这些蛋白不会被降解，这表明它们定位于病毒颗粒内部。在转染实验中，pIRS1和pTRS1适度激活了含有病毒主要立即早期启动子的报告质粒的表达，但不影响携带irs1/trs1立即早期启动子的报告基因的活性。这两个报告基因都被同样存在于病毒粒子中的pIRS1或pTRS1与pUL69的组合激活。
