Baleydier C, Achache P, Froment J C
INSERM, Unité 94, Bron, France.
Neuroreport. 1997 May 6;8(7):1691-6. doi: 10.1097/00001756-199705060-00026.
Monoclonal antibody (SMI-32) to neurofilament protein was used in an immunochemical study of the premotor cortex of two human brains in comparison with other architectonic techniques such as Nissi, myelin, cytochrome-oxidase and acetylcholinesterase staining in order to distinguish cortical subdivisions. SMI-32 immunoreactivity technique provides 'neurofilament architecture' patterns specific to area 4, caudal area 6 (area 6c) and rostral area 6 (area 6r). Particularly, the distinction between the two subdivisions of area 6, which is difficult to appreciate with the usual cytochemical or enzyme architectonic techniques, appears very apparent with this technique. Hence, it was possible to localize the topographic boundaries of area 6a alpha and 6a beta of the Vogts on the dorso-lateral convexity and the supplementary motor area and the presupplementary motor area on the mexial wall of the hemisphere.
为了区分皮质亚区，将抗神经丝蛋白单克隆抗体（SMI-32）用于对两例人类大脑运动前区皮质的免疫化学研究，并与其他组织学技术（如尼氏染色、髓磷脂染色、细胞色素氧化酶染色和乙酰胆碱酯酶染色）进行比较。SMI-32免疫反应技术可提供特定于4区、尾侧6区（6c区）和嘴侧6区（6r区）的“神经丝结构”模式。特别是，6区的两个亚区之间的区分，用常规细胞化学或酶组织学技术很难辨别，但用该技术却显得非常明显。因此，有可能在背外侧凸面定位沃格特6aα区和6aβ区的地形边界，以及在半球内侧壁定位辅助运动区和前辅助运动区。
