Wood B E, Aldrich H C, Ingram L O
Department of Microbiology and Cell Science, University of Florida, Gainesville 32611, USA.
Biotechnol Prog. 1997 May-Jun;13(3):232-7. doi: 10.1021/bp970027v.
The commercial production of ethanol from cellulose by simultaneous saccharification and fermentation (SSF) is prevented in part by the high cost of fungal cellulase enzymes. Intermittent exposure of SSF processes to ultrasonic energy under selected conditions (5 FPU of cellulase/g of substrate; 15 min of exposure/240 min cycle during the latter half of SSF) was found to increase ethanol production from mixed waste office paper by approximately 20%, producing 36.6 g/L ethanol after 96 h (70% of the maximum theoretical yield). Without ultrasound, 10 FPU of cellulase/g of substrate was required to achieve similar results. Continuous exposure of the organism to ultrasonic energy was bacteriostatic and decreased ethanol production but may be useful for the controlling bacterial growth in other processes.
纤维素酶成本高昂，这在一定程度上阻碍了通过同步糖化发酵（SSF）从纤维素商业生产乙醇。研究发现，在特定条件下（纤维素酶用量为5 FPU/g底物；在SSF后半程，每240分钟循环中暴露15分钟），让SSF过程间歇性暴露于超声能量下，可使混合办公废纸的乙醇产量提高约20%，96小时后乙醇产量达到36.6 g/L（为最大理论产量的70%）。若不使用超声，每克底物需要10 FPU的纤维素酶才能达到类似结果。让生物体持续暴露于超声能量下具有抑菌作用，会降低乙醇产量，但在控制其他过程中的细菌生长方面可能有用。
