Milanowski J
Katedry i Kliniki Chorób Płuc i Gruźlicy, Akademii Medycznej w Lublinie.
Pneumonol Alergol Pol. 1996;64 Suppl 1:78-89.
Mechanisms of chemotaxis of alveolar macrophages (AMs) and neutrophils (PMNs) in response to microbial products derived from organic dust were studied using blindwell chemotaxis chamber technique. Seven different agents (extract and endotoxin of Erwinia herbicola, extracts from Thermoactinomyces vulgaris and Aspergillus fumigatus, thermophilic protease and two preparations of glucans), were used for experiments. These agents were evaluated for their ability to direct attraction of AMs and PMNs and stimulation of alveolar macrophages to release chemotactic factors for other AMs and PMNs. The microbial products were able to attract both AMs and PMNs directly in a dose-dependent manner and the exposure of cultured AMs to most agents were stimulatory for production of chemotactic activity for AMs and PMNs. The generation and release of this activity by AMs may provide a mechanisms for the initiation and amplification of inflammatory reactions in the lung after inhalation of organic dust. Results of these in vitro studies may be relevant to the pathogenesis of alveolitis in organic dust-induced lung diseases.
采用盲孔趋化室技术研究了肺泡巨噬细胞（AMs）和中性粒细胞（PMNs）对源自有机粉尘的微生物产物作出反应时的趋化机制。使用了七种不同的试剂（草生欧文氏菌的提取物和内毒素、普通嗜热放线菌和烟曲霉的提取物、嗜热蛋白酶以及两种葡聚糖制剂）进行实验。评估了这些试剂直接吸引AMs和PMNs以及刺激肺泡巨噬细胞释放针对其他AMs和PMNs的趋化因子的能力。微生物产物能够以剂量依赖的方式直接吸引AMs和PMNs，并且将培养的AMs暴露于大多数试剂下会刺激其产生针对AMs和PMNs的趋化活性。AMs产生和释放这种活性可能为吸入有机粉尘后肺部炎症反应的启动和放大提供一种机制。这些体外研究结果可能与有机粉尘所致肺部疾病中肺泡炎的发病机制相关。
