Guerry P, Perez-Casal J, Yao R, McVeigh A, Trust T J
Enteric Diseases Program, Naval Medical Research Institute, Bethesda, Maryland 20889, USA.
J Bacteriol. 1997 Jun;179(12):3997-4002. doi: 10.1128/jb.179.12.3997-4002.1997.
Two genes involved in iron utilization in Campylobacter coli VC167 T1 have been characterized. The cfrA gene encodes a protein with a predicted Mr of 77,653 which, after processing of the leader sequence, has a predicted Mr of 75,635. This protein has significant sequence identity to siderophore receptors of several bacteria, and site-specific mutants defective in cfrA do not synthesize one of two major iron-repressible outer membrane proteins. An adjacent gene encodes a TonB-like protein; a mutant in this gene lost the ability to utilize hemin, ferrichrome, and enterochelin as iron sources. The cfrA and tonB genes of VC167 T1 hybridized to all strains of C. coli and most strains of C. jejuni examined but did not hybridize to several other strains of C. jejuni, suggesting that the thermophilic campylobacters can be separated into two categories based on the presence of these two iron utilization genes.
已对空肠弯曲菌VC167 T1中参与铁利用的两个基因进行了表征。cfrA基因编码一种预测分子量为77,653的蛋白质，在去除前导序列后，预测分子量为75,635。该蛋白质与几种细菌的铁载体受体具有显著的序列同一性，cfrA基因缺陷的位点特异性突变体不会合成两种主要的铁抑制性外膜蛋白之一。一个相邻基因编码一种类TonB蛋白；该基因突变体丧失了利用血红素、铁载体和肠螯合素作为铁源的能力。VC167 T1的cfrA和tonB基因与所有空肠弯曲菌菌株以及大多数检测的空肠弯曲杆菌菌株杂交，但不与其他几种空肠弯曲杆菌菌株杂交，这表明基于这两个铁利用基因的存在，嗜热弯曲杆菌可分为两类。
