Alobaidy Hussein Ali Kamil, Ayvaz Abdurrahman, Sağiroğlu Pınar, Atalay Mustafa Altay
Graduate School of Natural and Applied Science, Erciyes University, Kayseri, Turkey.
Department of Biology, Faculty of Science, Erciyes University, Kayseri, Turkey.
Indian J Microbiol. 2024 Sep;64(3):1000-1008. doi: 10.1007/s12088-024-01199-5. Epub 2024 Feb 13.
This study aimed to reveal antibiotic resistance patterns and molecular characterization of quinolone resistance isolates in patients with diarrhea. spp. isolated from 35.33% of the total samples, most of which were from male patients aged 3 months to 10 years. Identifying isolates at the species level made in MALDI-TOF MS, 82.4% were , and 17.6% were . Respectively 94% (47/50), 58% (29/50), and 2% (1/50) resistance rates were determined for ciprofloxacin, tetracycline, and erythromycin. While isolates were more resistant to ciprofloxacin and tetracycline than , they showed no resistance to erythromycin. Quinolone resistance determining region (QRDR) were evaluated by mismatch amplification mutation test and all quinolone resistant strains gave positive results. One of the seven silent mutations identified was specific to this study, and two other novel mutations were also identified.
The online version contains supplementary material available at 10.1007/s12088-024-01199-5.
本研究旨在揭示腹泻患者中喹诺酮耐药分离株的抗生素耐药模式和分子特征。从35.33%的总样本中分离出的物种，其中大多数来自3个月至10岁的男性患者。通过基质辅助激光解吸电离飞行时间质谱（MALDI-TOF MS）在物种水平鉴定分离株，82.4%为[具体物种1]，17.6%为[具体物种2]。环丙沙星、四环素和红霉素的耐药率分别为94%（47/50）、58%（29/50）和2%（1/50）。虽然[具体物种1]分离株比[具体物种2]对环丙沙星和四环素更耐药，但它们对红霉素无耐药性。通过错配扩增突变试验评估喹诺酮耐药决定区（QRDR），所有喹诺酮耐药菌株均呈阳性结果。鉴定出的七个沉默突变之一是本研究特有的，还鉴定出另外两个新突变。
在线版本包含可在10.1007/s12088-024-01199-5获取的补充材料。
