Korhonen T K, Lähteenmäki K, Kukkonen M, Pouttu R, Hynönen U, Savolainen K, Westerlund-Wikström B, Virkola R
Department of Biosciences, University of Helsinki, Finland.
Adv Exp Med Biol. 1997;412:185-92.
We evaluated in vitro the hypothesis that bacterial adhesiveness to the mammalian extracellular matrix and the activation of plasminogen on bacterial plasminogen receptors promote bacterial penetration through basement membranes. We used the strain SH401 of Salmonella enterica serovar Typhimurium, which adheres to the high-mannose chains of laminin, a major glycoprotein of basement membranes, and expresses plasminogen receptors. Bacterium-bound plasmin was able to degrade laminin and extracellular matrix preparations as well as to potentiate the penetration of bacteria through a reconstituted basement membrane. The results suggest that metastatic tumour cells and bacterial pathogens use similar mechanisms to penetrate through tissue barriers.
细菌对哺乳动物细胞外基质的黏附性以及细菌纤溶酶原受体上纤溶酶原的激活促进细菌穿透基底膜。我们使用了鼠伤寒沙门氏菌血清型鼠伤寒杆菌的SH401菌株，该菌株可黏附于基底膜的主要糖蛋白层粘连蛋白的高甘露糖链，并表达纤溶酶原受体。结合细菌的纤溶酶能够降解层粘连蛋白和细胞外基质制剂，并增强细菌穿透重组基底膜的能力。结果表明，转移性肿瘤细胞和细菌病原体利用相似的机制穿透组织屏障。
