Dozmorov I M, Prokhazova A L, Svirtshevskaya E V, Lutsan N I, Kozin I I, Lemenovskaya A F, Dyatlovitskaya E V, Bergelson L D
Shemyakin Institute of Bioorganic Chemistry, Ul. Miklukho-Maklaya, Moscow, Russia.
Biochem Mol Biol Int. 1997 Jun;42(1):57-63. doi: 10.1080/15216549700202431.
Exogenous gangliosides act as immunosuppressors when applied at micromolar concentrations corresponding to their average level in human plasma. Here we show that at nanomolar concentrations the gangliosides GD3, GD1a and GM1 can act as immunostimulators markedly enhancing the number of plaque-forming cells in mouse splenocyte culture responding to sheep erythrocytes. At such low concentration these gangliosides as well as GM3 were not able to influence significantly proliferative responses of splenic B and T lymphocytes or of cytotoxic T-cells. Neither did they change significantly the production of IL-1 by antigen- representing cells, or of IL-2 by Con A-induced blasts in the splenocyte culture. It is suggested that the stimulatory effect of low ganglioside concentrations on humoral response is due to their influence on cooperative cell-cell interactions required for the differentiation of B-cells into Ig-secreting cells.
当以与人类血浆中平均水平相对应的微摩尔浓度应用时，外源性神经节苷脂可作为免疫抑制剂。在此我们表明，在纳摩尔浓度下，神经节苷脂GD3、GD1a和GM1可作为免疫刺激剂，显著增加小鼠脾细胞培养物中对绵羊红细胞产生反应的噬斑形成细胞数量。在如此低的浓度下，这些神经节苷脂以及GM3均无法显著影响脾B淋巴细胞、T淋巴细胞或细胞毒性T细胞的增殖反应。它们也没有显著改变抗原呈递细胞产生IL-1的情况，或脾细胞培养物中Con A诱导的母细胞产生IL-2的情况。有人提出，低浓度神经节苷脂对体液反应的刺激作用是由于它们对B细胞分化为分泌Ig的细胞所需的细胞间协同相互作用产生了影响。
